Magnetically-labeled biological sensor as well as production method and detection method thereof

Abstract

The invention discloses a magnetically-labeled biological sensor. A bioprobe fixing layer of the magnetically-labeled biological sensor comprises a conducting layer, an insulating layer and a power supply device, wherein a plurality of conducting units are embedded into the surface of the insulating layer; each conducting unit is electrically connected with the conducting layer; the conducting units form a conducting unit array; when the power supply device is used for supplying power, the magnetically-labeled bioprobe is fixedly adsorbed on the surface of the conducting unit array; after detection, the magnetically-labeled bioprobe is immediately separated from the conducting unit array by stopping power supply; and the magnetically-labeled bioprobe can be thoroughly removed by cleaning.Therefore, the phenomenon that the recycle of a chip is influenced because of the magnetically-labeled bioprobe which cannot be effectively removed after detection caused by fixation of the bioprobe by adopting a chemical coupling method in the prior art is avoided; meanwhile, traditional detection precision can be maintained; and the magnetically-labeled biological sensor is a novel magnetically-labeled biological sensor which has the advantages of simple structure, convenience in use, capability of being repeatedly used and potential application value.

Description

technical field [0001] The invention relates to the field of labeling and detection of biomolecules, in particular to a magnetic label biosensor, a preparation method and a detection method thereof. Background technique [0002] Biosensors generally include two parts: one is a biological probe that can specifically recognize the molecule to be tested; the other is a transducer that can convert biological signals into physical signals such as light, electricity, and magnetism. Generally speaking, the optical, electrical, and magnetic properties of biomolecules are very weak. Therefore, in the detection process of the molecules to be detected, it is necessary to assist sensitive materials such as light and magnetism, that is, to label them, so that the molecules to be detected can be detected relatively easily. Among them, the magnetic labeling technology is a new immunological technology that has been researched more popular at home and abroad in recent years. It is a magneti...

AI Technical Summary

Problems solved by technology

However, due to the binding force between the biological probe 4 and the biological probe fixed layer 3, the binding force between the target molecule 5 and the biological probe 4, and the binding force between the magnetic particle 6 and the target molecule 5, only relying on the gradient magnetic field The method cannot effectively remove the biological probes 4 and magnetic microspheres 6 on the surface of the magnetically labeled biosensor chip, thereby affecting the reuse of the magnetically labeled biosensor chip

Images