Preparation method for avian pasteurella genome vaccine

Abstract

The invention discloses a preparation method for an avian pasteurella genome vaccine. The preparation method comprises the following steps of: performing enzyme digestion on a target gene by using a restrictive incision enzyme Sau3AI; performing enzyme digestion on an eucaryon expression vector PcDNA 3.1(+) by using a restrictive incision enzyme BamHI; performing dephosphorylation on the eucaryon expression vector PcDNA 3.1(+) which is subjected to enzyme digestion by using calf intestinal alkaline phosphatase; connecting an avian pasteurella genome with the eucaryon expression vector pcDNA3.1(+); transform into a colon bacillus competence JM83; extracting plasmids; and identifying through enzyme digestion to obtain the avian pasteurella genome vaccine. As proved by animal experiment detection, the avian pasteurella genome vaccine prepared with the method can be used for reducing the generation of avian pasteurellosis and reducing the invasion of avian pasteurella to birds; and the preparation method is simple and is easy to operate.

Description

technical field [0001] The invention relates to a preparation method of a bacterial vaccine, in particular to a preparation method of an avian Pasteurella genome vaccine. Background technique [0002] Pasteurella avian (also known as Pasteurella multocida) is the pathogenic bacterium of fowl cholera (also known as avian pasteurellosis), and the fowl cholera disease caused by this bacteria is one of the more common poultry infectious diseases in my country , almost all poultry can be infected with the disease, and the morbidity and mortality of poultry infected with the disease are very high, with severe diarrhea, acute onset, short course of disease, and fast death as the main features. The nationwide prevalence of the disease has seriously affected the healthy and stable development of my country's poultry industry and brought huge economic losses to poultry farmers. [0003] Genomic vaccines are recombinant eukaryotic expression plasmids with antigen genes, which are used ...

AI Technical Summary

Problems solved by technology

[0004] At present, the vaccines used in my country to prevent poultry cholera caused by Pasteurella avian mainly include attenuated live vaccines and inactivated vaccines. Among them, attenuated live vaccines are the most studied type of attenuated live vaccines for all poultry infectious diseases in my country. In the 1980s, it reached the peak period of development, but due to the inherent problems of Pasteurella avium's complex antigen structure and easy mutation, an ideal attenuated live vaccine has not yet been obtained.

There are a series of problems in the existing commercially available attenuated live vaccines, such as low immune protection, short immune period, large local and systemic reactions, resulting in food loss and serious drop in egg production, and sometimes even partial vaccination. The death of poultry, as well as technical problems such as insufficient genetic stability of the strain, improper application may even cause outbreaks of fowl cholera, so the effect is not very satisfactory

The problems of low protection rate and short immunity period of inactivated vaccines are more serious than attenuated live vaccines, so it is imperative to develop new and effective avian pasteurellosis vaccines

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