Method for rapidly breeding colored-leaf clove by utilizing tissue culture

Abstract

The invention discloses a method for rapidly breeding colored-leaf clove by utilizing tissue culture. The method is characterized by comprising the following steps: (1) collecting and sterilizing an explant; (2) inoculating a stem section obtained in the previous step to an explant induction culture medium and inducing for 30 days until the gemmule length is 4-5cm; (3) transferring a young seedling obtained in the previous step into a successive transfer culture medium and culturing for 20 days; (4) transferring the unrooted young seedling with the length of 2-3cm into a rooting culture medium and culturing for 40 days; (5), after placing a culture bottle in a greenhouse and carrying out seeding based on natural light for 2-3 days, taking out a rooted young seedling and rinsing the rootedyoung seedling in a 0.1% potassium permanganate solution; and (6) planting the rinsed rooted young seedling in a culture medium, and transplanting the young seedling to a field seedling nursery for planting when the young seedling grows to 20-30cm. In the process selected in the invention, three aspects including improving the propagation quantity, maintaining the hereditary characters and reducing vitrification are considered, and the rapid propagation of colored-leaf clove is realized through batch tissue culture.

Description

technical field [0001] The invention relates to plant seedling propagation technology, in particular to a method for rapidly propagating lilac cloves by using tissue culture. Background technique [0002] Coleus lilac (Syringa vulgaris L.) leaves are spotted with gold sprinkles that stand out in sunlight. It has a well-developed root system, vigorous plant growth, strong sprouting ability and drought resistance, and leaves fall late in winter. It is a rare tree species for urban greening and beautification. However, as a new variety introduced from Europe in recent years, the number of cuttings and grafting available is small, which not only limits its reproduction speed, but also is not conducive to the maintenance of its excellent traits, so it cannot meet the increasing social needs. It is an effective way to solve this production problem by using plant tissue culture technology to industrialize the production of high-quality Lilac seedlings. However, like other woody p...

AI Technical Summary

Problems solved by technology

However, like other woody plants, vitrification is one of the difficult problems to be solved in its tissue culture, and for ornamental color-leaved tree species such as Coleus lilac, because there are many genetic chimeras, such as some mosaic color Seeds of golden-edged, silver-edged, mosaic plants, during the cultivation process, the components of the chimera may separate, so that the variegated plants will show altered morphology or completely lose the variegated shape, thereby further increasing the cultivation difficulty

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