Regular disinfection method for continuous unicellular alga culture system and vapor disinfection system thereof

A technology of a culture system and a sterilization method, which is applied in the field of regular sterilization methods and its steam sterilization system, can solve the problems of inability to sterilize methods, sterilization, and large volume of plastic barrels or plastic tanks, so as to save manpower, material resources and power consumption, and improve efficiency. Effect

Inactive Publication Date: 2013-09-11
OCEAN UNIV OF CHINA +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When expanding the culture, the amount of cultured single-celled algae is relatively large, and the volume of the plastic barrel or plastic tank is relatively large. It cannot be sterilized in a pressure cooker or an oven. It can only be sterilized with chemical reagents such as bleaching powder after washing.
However, during productive cultivation, the cultured water body is very large, and the devices or containers used are also large, let alone laboratory disinfection methods, but chemical reagents can only be used for disinfection.

Method used

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  • Regular disinfection method for continuous unicellular alga culture system and vapor disinfection system thereof
  • Regular disinfection method for continuous unicellular alga culture system and vapor disinfection system thereof

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Embodiment

[0045] Such as figure 1 As shown, after each batch of unicellular algae has been cultivated, at first the continuous filling culture solution pipeline 3 in the culture system is communicated with the continuous collection pipeline 6 of the algae liquid with the U-shaped glass tube 9; Filling culture solution pipeline 3 and the branch of algae liquid continuous collection pipeline 6 are closed to form a pipeline to be sterilized;

[0046] The steam storage device 111 is connected to the input end of the continuous filling culture solution pipeline 3, so that the steam generated by the steam blowing device 113 is input into the above-mentioned interconnected continuous filling culture solution pipeline 3 and the continuous collection pipeline of algae liquid through the steam delivery pipeline 110 6 for steam sterilization;

[0047] Then use the heated and sterilized water to rinse the connected continuous filling culture solution pipeline 3 and the algae liquid continuous coll...

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Abstract

The invention discloses a regular disinfection method for a continuous unicellular alga culture system and a vapor disinfection system thereof. The method comprises the following steps of: after culturing of every batch of unicellular algae is completed, communicating a culture solution filling pipeline with an alga collecting pipeline through a U-shaped glass pipe to form a pipeline to be disinfected; performing first flushing on the pipeline to be disinfected by using heated and disinfected water; inputting softened water into a vapor generating device for generating vapor; and conveying the vapor into the pipeline needing to be disinfected with vapor by using a vapor blowing device through a pipeline for disinfecting with vapor. The vapor disinfection system has the advantages of high safety, high vapor generating efficiency, large disinfection range, control over water, electricity and vapor, and real-time grasp of the water level, vapor pressure and running condition. Due to the adoption of the method and the system, the problem of the absence of a relevant disinfection method and equipment in the prior art is solved, the system can be disinfected effectively during culturing of each batch of unicellular algae, a sterile environment for the continuous culture process is ensured, and the yield of unicellular algae is greatly increased.

Description

technical field [0001] The invention relates to a disinfection method and system, in particular to a regular disinfection method for a continuous culture system of unicellular algae and a steam disinfection system thereof, which is suitable for sterilizing the continuous culture system of unicellular algae. Background technique [0002] The growth of unicellular algae has certain requirements for the water body environment, and when the high-density continuous culture of unicellular algae is carried out, this requirement is even higher, and a very strict aseptic environment is required. Whether the environment for cultivating unicellular algae is suitable is not only related to whether the water body to be cultivated is strictly disinfected and whether the space is isolated from the outside world, but also closely related to the disinfection of the system pipeline after each batch of cultivation. In order to achieve high-density continuous culture of Unicellular algae, it is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L2/07
Inventor 赫勇梁英宫庆礼邓志科周丽刘明
Owner OCEAN UNIV OF CHINA
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