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Production method for astaxanthin-containing composition

A technology of astaxanthin and composition, applied in the field of composition preparation, can solve the problems of weak red color, a lot of time and labor, and randomness and the like

Active Publication Date: 2012-05-23
KANEKA CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the red color of colonies such as mutant strains low in astaxanthin content is weakened, it is very difficult to select target strains based only on the colony color, and the achievement of the target is subject to chance, and requires a lot of time and labor

Method used

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  • Production method for astaxanthin-containing composition
  • Production method for astaxanthin-containing composition
  • Production method for astaxanthin-containing composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] [Example 1] Sulfuric acid treatment of cell extract of Xanthophyllomyces dendrorhous KNK-03 strain

[0067] Put 10 mg of the cell extract obtained in Preparation Example 2 into a sealable test tube, add 3 ml of a sulfuric acid aqueous solution of the concentration shown in Table 1 below, and stir. The pH of the stirred solution was measured with an F-22 pH meter (Horiba, Ltd.).

[0068] After shaking each test tube at 30, 50, and 70°C for 2 hours, 2 ml of chloroform was added and stirred to dissolve the bacterial extract. After centrifugal separation, the aqueous phase was removed to obtain a chloroform phase containing the processed cell extract. After diluting it 10 times with acetone, it was submitted to HPLC analysis, and the relative amount of HDCO equivalent to astaxanthin in the cell extract after each treatment was calculated.

[0069] The relative amount of HDCO equivalent to astaxanthin is calculated according to the following formula:

[0070] The relative amount o...

Embodiment 2

[0076] [Example 2] Hydrochloric acid treatment of cell extracts of Phaffia rhodozyma KNK-03 strain

[0077] Except that hydrochloric acid was used instead of sulfuric acid, it was carried out in the same manner as in Example 1. The results are shown in Table 3.

[0078] [table 3]

[0079]

[0080] Table 3 shows that by contacting the cell extract of Phaffia rhodozyma KNK-03 strain containing astaxanthin and HDCO with hydrochloric acid, the relative amount of HDCO relative to astaxanthin in the bacterial extract is reduced. In addition, according to the results of Table 2 and Table 3, the effect of the present invention has nothing to do with the type of acidic medium.

Embodiment 3

[0081] [Example 3] Sulfuric acid treatment of partially purified cell extract of Phaffia rhodozyma KNK-03 strain

[0082] It was carried out in the same manner as in Example 1, except that the partially purified product obtained in Preparation Example 3 was used instead of the cell extract obtained in Preparation Example 2. The results are shown in Table 4.

[0083] [Table 4]

[0084]

[0085] Table 4 shows that by contacting a partial purified product of the cell extract of Phaffia rhodozyma KNK-03 strain containing astaxanthin and HDCO with sulfuric acid, the relative ratio of HDCO to astaxanthin in the bacterial extract is reduced the amount.

[0086] According to the results in Table 2 and Table 4, it can be seen that regardless of the concentration of the two compounds in the composition containing astaxanthin and HDCO, the method of the present invention is effective.

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Abstract

Disclosed is a method for reducing the amount of 3-hydroxy-3',4'-didehydro-beta-Psi-caroten-4-one (HDCO) relative to astaxanthin in an astaxanthin and HDCO containing composition by bringing the composition into contact with a basic medium having a pH of 9 or more and / or an acid medium having a pH of 3 or less. Also disclosed is an astaxanthin-containing composition production method, which includes a step whereby the relative amount of HDCO is reduced using the above method. By means of the disclosed methods, the relative amount of HDCO, the biological functions of which are unknown, contained in an astaxanthin-containing composition can be easily reduced.

Description

Technical field [0001] The invention relates to a preparation method of a composition containing astaxanthin. In particular, it relates to the relative ratio of HDCO in astaxanthin-containing compositions containing 3-hydroxy-3′,4′-didehydro-β,Ψ-carotene-4-one (HDCO) to astaxanthin Method of reducing the amount. Background technique [0002] Astaxanthin is a naturally occurring carotenoid that can be widely used as a feed additive for improving the color of the meat and skin of farmed fish. At the same time, it has attracted much attention in recent years as a functional food raw material. [0003] It is known that astaxanthin belongs to Xanthophyllomyces (formerly known as Phaffia), Brevundimonas, Haematococcus, Chlamydomonas ), Monoaphidium, Erythrobacter, Agrobacterium, Paracoccus, Labyrinthulea and other microorganisms, and can also be produced by chemical Synthetic preparation. [0004] 3-Hydroxy-3',4'-didehydro-β,Ψ-carotene-4-one (hereinafter referred to as HDCO) is one of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00A23K1/16A23L1/30A61P3/00A61K31/01A61K31/122A61K35/74C12R1/01C12R1/645
CPCA23K1/1603A61K31/01C12P23/00A23K1/1606A61K31/122A23K20/174A23K20/179A61P3/00
Inventor 井上博晶木崎宪之难波弘宪
Owner KANEKA CORP