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Device and method for non-specific rapid enrichment and purification of bacteria in liquid sample

A non-specific, purification device technology, applied in biochemical cleaning devices, biochemical equipment and methods, methods of stress-stimulated microbial growth, etc., to achieve the effects of high enrichment and purification efficiency, flexible use and wide application range

Active Publication Date: 2012-06-20
SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The principle of adsorption is still inconclusive, it may be due to the non-specific binding of magnetic beads to bacterial surface proteins, or due to the 3 o 4 The particles are positively charged, and the surface of the bacteria is negatively charged due to the presence of teichoic acid, and the two are combined by electrostatic attraction

Method used

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  • Device and method for non-specific rapid enrichment and purification of bacteria in liquid sample
  • Device and method for non-specific rapid enrichment and purification of bacteria in liquid sample
  • Device and method for non-specific rapid enrichment and purification of bacteria in liquid sample

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Embodiment Construction

[0038] In order to further understand the invention content, characteristics and effects of the present invention, the following examples are given, and detailed descriptions are as follows in conjunction with the accompanying drawings:

[0039] See figure 1 , a non-specific rapid enrichment and purification device for bacteria in a liquid sample, the device includes a control system and a flow system, wherein the control system is mainly composed of a peristaltic micropump 6, a three-way valve A5, a three-way valve B10, and a three-way valve C13 , a heating block 16, a temperature sensor 15, an electromagnet 8 and a main control module 7; The pipeline 17 of the assembly is composed. In this embodiment, the single maximum sample processing volume is designed to be 20ml. In this embodiment, the adsorption column 3, the three-way valve A5, the peristaltic micropump 6, the magnetic separation column 9, the three-way valve B10, and the three-way valve C13 are connected end to en...

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Abstract

The invention discloses a device and a method for non-specific rapid enrichment and purification of bacteria in a liquid sample. The device comprises a control system and a flow path system, wherein the flow path system comprises an adsorption column, a buffer solution bottle, a magnetic separation column, a sample discharge pipe, a waste liquid bottle and a pipe communicated with the individual components; a three-way valve A is connected with the buffer solution bottle, a three-way valve B is connected with the sample discharge pipe, and a three-way valve C is connected with the waste liquid bottle; a heating block is arranged at the bottom of the adsorption column; a temperature sensor is attached on the bottom wall of the inner cavity of the adsorption column; the magnetic separation column is surrounded by the poles of an electromagnet; and a main control module controls a peristaltic micropump, the three-way valves A, B and C, the heating block and the electromagnet. The invention also discloses the method for implementing non-specific rapid enrichment and purification of bacteria in a liquid sample by using the device mentioned above. The device and the method can achieve the effect of non-specific rapid enrichment and purification of bacteria in liquid samples.

Description

technical field [0001] The present invention relates to sample pretreatment technology in related fields such as sanitation and safety quarantine, environmental monitoring, etc., in particular to a device and method for non-specific rapid enrichment and purification of bacteria in liquid samples. Background technique [0002] Detection of specific indicator bacteria, especially pathogenic bacteria, is an important task in the fields of health and safety quarantine and environmental monitoring. In practical applications such as food hygiene safety and environmental water quality monitoring, the content of indicator bacteria in the prepared or collected original liquid samples (mainly aqueous solution samples) is usually low, but the background impurities are very complicated, and the existing detection methods are difficult to detect directly. It must be enriched and purified in advance. Therefore, the enrichment and purification of bacteria in liquid samples has become a ke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42C12M1/38C12M1/36C12N1/20C12N13/00
CPCC12M47/12C12M35/02C12M41/12C12M35/06C12M47/10
Inventor 杜耀华吴太虎陈锋程智顾彪
Owner SANITARY EQUIP INST ACAD OF MILITARY MEDICAL SCI PLA
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