System and method for simultaneous detection of extracellular environmental toxicants and cell metabolism

A technology of cell metabolism and external environment, which is applied in the field of detection system for detecting extracellular environmental toxicants and cell metabolism at the same time, can solve the problem of being unable to quantitatively study extracellular heavy metal ions, unable to give quantitative relationships, hindering the relationship between extracellular environmental toxicants and Analyze the rapid development of cell metabolism mechanism and other issues to achieve good sealing, improve service life, and reduce the cost of replacement parts

Inactive Publication Date: 2012-06-27
ZHEJIANG UNIV
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Problems solved by technology

However, there is no sensor and detection method that can simultaneously detect the concentration of heavy metal ions in the extracellular environment and the concentration of cellular metabolic ions. Therefore, it is impossible to quantitatively study the effect of the concentra

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  • System and method for simultaneous detection of extracellular environmental toxicants and cell metabolism
  • System and method for simultaneous detection of extracellular environmental toxicants and cell metabolism
  • System and method for simultaneous detection of extracellular environmental toxicants and cell metabolism

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Embodiment Construction

[0020] The basic principle of the photo-addressable potentiometric sensor used and the Zn 2+ Chalcogenide glass sensitive film, Cl - The basic principle of polyvinyl chloride sensitive membrane to detect ions.

[0021] The core of the optical addressable potential sensor is the electrolyte / insulating layer / semiconductor structure. The optical addressable potential sensor uses the internal photoelectric effect of the semiconductor, that is, when the semiconductor is irradiated by light of a certain wavelength, the semiconductor absorbs photons, and the forbidden band occurs. The transition produces electron-hole pairs. When a bias voltage is applied to both sides of the sensor, a depletion layer is generated in the semiconductor, and some of these photogenerated carrier pairs can always reach the depletion layer before recombination, so the strong electric field of the depletion layer for electrons and holes Separation, the movement of electron-hole pairs in this process form...

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Abstract

The invention discloses a system and method for simultaneous detection of extracellular environmental toxicants and cell metabolism. In the invention, a Zn<2+> chalcogenide glass sensitive membrane and a Cl<-> polyvinyl chloride sensitive membrane simultaneously deposit and combine on the surface of a light-addressable potentiometric sensor so as to form a Zn<2+> chalcogenide glass sensitive membrane-Cl<-> polyvinyl chloride sensitive membrane-light-addressable potentiometric sensor, which can quickly detect the Zn<2+> concentration of extracellular environmental toxicants and the Cl<-> ion concentration of cell metabolism at the same time, and infer the quantitative relationship between the two. A constructed micro-cavity can improve the sensitivity of ion concentration, and can ensure the consistency of detection conditions simultaneously, thus greatly reducing the part replacement cost and improving the service life of a sensor package unit. The above characteristics can meet the requirements for simultaneous detection of extracellular environmental toxicants and cellular metabolism, so that the system and method of the invention can be widely used in related fields of researchon heavy metal concentration and cell metabolism mechanism.

Description

technical field [0001] The invention relates to a detection technology, in particular to a detection system and a detection method for simultaneously detecting extracellular environmental poisons and cell metabolism. Background technique [0002] There are a wide variety of extracellular environmental toxicants, such as bacteria, viruses, toxic drugs, heavy metal poisons, etc., and sensors and detection methods for detecting these environmental toxicants have also been developed. At the same time, cell metabolism sensors and detection methods are also developing rapidly, such as patch clamp method, amperometric method, membrane capacitance method, etc. These technologies are also developing towards commercialization. However, there is no sensor and detection method that can simultaneously detect the concentration of heavy metal ions in the extracellular environment and the concentration of cellular metabolic ions. Therefore, it is impossible to quantitatively study the effec...

Claims

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Application Information

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IPC IPC(8): C12M1/34G01N27/26C12Q1/02
Inventor 王平胡宁哈达吴成雄程功王天星王江容周洁
Owner ZHEJIANG UNIV
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