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Method for separating and purifying sea cucumber polysaccharide, and application of two aqueous phase extraction method to method for separating and purifying sea cucumber polysaccharide

A sea cucumber polysaccharide, separation and purification technology, applied in the field of separation and purification, to achieve the effect of mild conditions, low equipment requirements, and easy scale-up

Active Publication Date: 2013-12-18
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no research reports or patents on the use of aqueous two-phase extraction technology for the separation and purification of sea cucumber polysaccharides.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 5g of polyethylene glycol with a weight average molecular weight of 10000 and 17g (NH 4 ) 2 SO 4 Dissolve in 78g of water, mix thoroughly and then stand to separate to form a two-phase aqueous system. Dissolve 0.2g sea cucumber polysaccharide in the two-phase aqueous system, fully dissolve, mix evenly, and then separate into layers, and take out the upper phase and the lower phase.

[0031] The upper phase was desalted by dialysis with a dialysis bag with a molecular weight cut-off of 50,000, and the dialysis retentate was freeze-dried to obtain sea cucumber fucoidan sulfate with a purity of 96.0%. The lower phase was dialysis desalted with a dialysis bag with a molecular weight cut-off of 14000, and the dialysis retentate was freeze-dried to obtain sea cucumber chondroitin sulfate with a purity of 97.1%.

Embodiment 2

[0033] 25g of polyethylene glycol with a weight average molecular weight of 10000 and 10g (NH 4 ) 2 SO 4 Dissolve in 65g of water, mix thoroughly and then stand to separate to form a two-phase aqueous system. Dissolve 0.4g sea cucumber polysaccharide in the two-phase aqueous system, fully dissolve, mix evenly, and then let stand to separate layers, and take out the upper phase and the lower phase.

[0034] The upper phase was desalted by dialysis with a dialysis bag with a molecular weight cut-off of 50,000, and the dialysis retentate was freeze-dried to obtain sea cucumber fucoidan sulfate with a purity of 97.3%. The lower phase was dialysis desalted with a dialysis bag with a molecular weight cut-off of 14000, and the dialysis retentate was freeze-dried to obtain sea cucumber chondroitin sulfate with a purity of 96.6%.

Embodiment 3

[0036] 7.5kg weight average molecular weight is the polyethylene glycol of 10000 and 6.25kg (NH 4 ) 2 SO 4 Dissolve in 36.25kg of water, mix thoroughly and then stand to separate to form a two-phase aqueous system. Dissolve 100g sea cucumber polysaccharide in the two-phase aqueous system, fully dissolve, mix and separate, and take out the upper phase and the lower phase.

[0037] The upper phase is desalted by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 100,000, and the ultrafiltration retentate is spray-dried to obtain sea cucumber fucoidan sulfate with a purity of 98.6%. The lower phase is desalted by ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 50,000, and the ultrafiltration retentate is spray-dried to obtain sea cucumber chondroitin sulfate with a purity of 98.1%.

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Abstract

The invention provides a method for separating and purifying sea cucumber polysaccharide. The method comprises the following steps of: 1, separating, namely performing two aqueous phase extraction on the sea cucumber polysaccharide to obtain an extraction upper phase and an extraction lower phase; and 2, performing aftertreatment, namely desalting the extraction upper phase obtained in the step 1 and drying to obtain fucoidan isolatedfrom sea cucumber, and desalting the extraction lower phase obtained in the step 1 and drying to obtain sea cucumber chondroitin sulfate so as to separate and purify the sea cucumber polysaccharide. A novel separation technology, namely the two aqueous phase extraction technology is applied to the separation and purification of the sea cucumber polysaccharide; and the method has the advantages of mild condition, convenience in operation, low requirement on equipment, and low cost; and amplification is easy to realize, a product is high in purity, and the defects in the conventional method for separating and purifying the sea cucumber polysaccharide are overcome.

Description

technical field [0001] The invention relates to the technical field of separation and purification, in particular to a method for separation and purification of sea cucumber polysaccharides. Background technique [0002] Sea cucumber polysaccharides are an important part of the body wall of sea cucumbers, which account for 5% to 10% of the dry weight of sea cucumber body walls. The sea cucumber polysaccharide is connected with the protein in the sea cucumber body wall, and the sea cucumber polysaccharide can be extracted from the sea cucumber body wall through protease hydrolysis, ethanol precipitation and other steps. Sea cucumber polysaccharide is a mixture, which mainly includes sea cucumber chondroitin sulfate and sea cucumber fucoidan sulfate. These two substances have different chemical structures and physiological activities respectively. The chemical structure of sea cucumber chondroitin sulfate is similar to that of chondroitin sulfate, which has been proven to hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08B37/08
Inventor 常耀光宿玮薛长湖续晓琪于龙李兆杰薛勇徐杰王玉明王静凤
Owner OCEAN UNIV OF CHINA