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Method for rapid propagating tissue of wistaria

A tissue culture fast propagation and wisteria technology, applied in the field of horticulture, can solve problems such as the limitation of reproduction speed and the difficulty of meeting the needs of garden applications, and achieve the effect of short generation cycle

Inactive Publication Date: 2013-12-04
TAICANG LINJIANG FARM SPECIALIZED COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wisteria is mostly propagated by cuttings and seeds, and its propagation speed is limited by the female parent material, which has certain difficulties in meeting the needs of garden applications.

Method used

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Examples

Experimental program
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Embodiment Construction

[0015] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0016] Embodiments of the invention include:

[0017] A wisteria tissue culture rapid propagation method, is characterized in that, comprises the following steps:

[0018] Step (1) Establishment of explants: in late April, take the vigorous and young stems of wisteria in the same year, disinfect with 1% washing powder solution, and soak in sterile water for 30 minutes;

[0019] Step (2) explant treatment: soak 60s with 70% alcohol solution, wash 2 times with sterile water, drain, then put into 0.1% HgCl 2 Soak in the solution for disinfection for 20 minutes, rinse with sterile water for 3 times, absorb the water with gauze, cut off the stems with leaf buds, and set aside;

[0020] Step (3) pri...

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Abstract

The invention relates to a method for rapid propagating tissue of wistaria, which is realized by the steps of explant selecting, cleaning, primary culturing, sub-culturing and rooting culturing, wherein a special culture medium is selected for the primary culturing, the sub-culturing and the rooting culturing, so that the purposes of high propagation coefficient and good propagation effect are achieved.

Description

technical field [0001] The invention relates to the field of horticulture, in particular to a method for tissue culture and rapid propagation of wisteria. Background technique [0002] Wisteria (scientific name: Wisteria sinensis (Sims) Sweet) belongs to the genus Wisteria of the Fabaceae family. It is a large deciduous climbing vine with dark gray dry skin and no cracking; the flowers are purple or dark purple, very beautiful. Wisteria is a plant in the warm and temperate zone. It has strong adaptability to climate and soil, is more cold-tolerant, can tolerate water humidity and poor soil, loves light, and is more shade-tolerant. Wisteria is native to my country, and is also distributed in Korea and Japan. It is mostly distributed in North China, and is most common in Hebei, Henan, Shanxi, and Shandong. It is cultivated in East China, Central China, South China, Northwest China and Southwest China. From Guangdong in the south to Inner Mongolia in the north, it is widely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 窦祥龙
Owner TAICANG LINJIANG FARM SPECIALIZED COOP