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Anti-tumor compound, and preparation method and application thereof

A compound and anti-tumor technology, applied in the field of anti-tumor compounds and their preparation, to achieve the effects of simple process, correct structure and mature extraction method

Inactive Publication Date: 2014-06-04
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the treatment of cancer is facing a very serious challenge

Method used

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  • Anti-tumor compound, and preparation method and application thereof
  • Anti-tumor compound, and preparation method and application thereof
  • Anti-tumor compound, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, the acquisition of compound LS1924A

[0058]Soluble starch was purchased from Beijing Chemical Plant, the product catalog number is K0800034; glucose was purchased from Beijing Modern Oriental Fine Chemical Co., Ltd. (HG). Millet flour was purchased from farmers' markets. Cottonseed protein powder (medium) was purchased from Beijing Zhongmian Ziguang Biotechnology Co., Ltd. MOPS, 3-(N-morpholino)propanesulfonic acid, was purchased from Beijing Jiangchen Biology. HP-20 macroporous adsorption resin (Mitsubishi): purchased from Beijing Huideyi Technology Co., Ltd. Agilent Zorbax XDB C-8column: purchased from Beijing Huideyi Technology Co., Ltd. Agilent ZORBAX XDB C-18column: purchased from Beijing Huideyi Technology Co., Ltd.

[0059] 1. Discovery and identification of Streptomyces sp. LS1924 CGMCC No.5240

[0060] The source sample of the strain: the soil sample collected from the primeval forest (850 meters above sea level) in Yaoli, Jiangxi.

[0061]...

Embodiment 2

[0120] Example 2, detection of antitumor activity of compound LS1924A

[0121] The MTT method was used to detect the survival inhibitory activity of the compound LS1924A obtained in Example 1 on human liver cancer HepG2 cells (purchased from the Medical Cell Center, Chinese Academy of Medical Sciences). Medical Cell Center, Chinese Academy of Medical Sciences), counted after digestion. 3×10 3 One per well (190 μl) was seeded in a 96-well cell culture plate. 24 hours after the cells adhere to the wall, add 10 μl of different concentrations of the sample solution to be tested, so that the final concentrations of the samples to be tested in each group of wells are 1 μg / ml, 2 μg / ml, 5 μg / ml, 10 μg / ml, 20 μg / ml and 50μg / ml, each group has at least 3 parallel wells, and treat the cells for the specified time. Add 20 μl of MTT solution to each well before measurement (MTT is made into a 5 mg / ml solution with sterile PBS buffer), incubate at 37°C for 4 hours, carefully suck off the...

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Abstract

The invention discloses an anti-tumor compound, and a preparation method and application thereof. According to the anti-tumor compound, a structural formula is shown as a formula I. The compound LS1924A belongs to a compound with a new structure, and has high anti-tumor activity, and is suitable for the study of anti-tumor lead compounds or the preparation of anti-tumor medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an antitumor compound and its preparation method and application. Background technique [0002] According to the statistics of the World Health Organization, cancer is a major cause of death worldwide, and the number of deaths from cancer worldwide is increasing year by year. In 2008, the number of cancer deaths reached 7.6 million (accounting for about 13% of all deaths) , is projected to increase global cancer deaths by 45% by 2030. Therefore, the treatment of cancer is facing very serious challenges. It is very important to discover and study anti-tumor drugs with high efficiency and low toxicity. Contents of the invention [0003] One object of the present invention is to provide a compound. [0004] The compound provided by the present invention has a structural formula as shown in formula I: [0005] [0006] Formula I. [0007] Another object of the present invention...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/08C12P17/18A61K31/357A61P35/00C12N1/20C12R1/465
Inventor 张立新宋福行陈彩霞郭徽刘雪婷代焕琴
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI