Selenized microbial exopolysaccharide, and preparation method and uses thereof
An extracellular polysaccharide and microorganism technology, which is applied in the field of selenized small-molecule microbial exopolysaccharide and its preparation, can solve the problems of long reaction time, complicated reaction process, complicated preparation process, etc., and achieves no toxic by-products and tumor inhibition. The effect of high rate and simple reaction process
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Embodiment 1
[0016] 1) Weigh 1.0 g of small molecule Caragana rhizobia exopolysaccharide (7.216kD) into the flask, then add 100 mL of 1% nitric acid solution to dissolve it, stir at room temperature for 12 hours; then weigh 0.5 g of sub Selenic acid and 0.6 g of barium chloride were added to the flask, stirred and heated at 60°C for 6 hours;
[0017] 2) Add dropwise 1.88 mL of 1mol / L sulfuric acid to the above reaction solution, and centrifuge for 15 minutes at 6000 rpm to remove the precipitate;
[0018] 3) Adjust the pH of the supernatant to 7 with sodium hydroxide, put it into a dialysis bag with a molecular weight cut-off of 3000Da, first dialyzed with tap water for 60 hours, then dialyzed with distilled water for 24 hours, until the dialysis is detected with 0.002mg / mL ascorbic acid solution The liquid outside the bag no longer turns red;
[0019] 4) Concentrate the dialysate to 1 / 2 of its original volume under reduced pressure at 60°C and 0.08Mpa, and add 150mL 95% edible alcohol to the co...
Embodiment 2
[0021] 1) Weigh 1.0 g of small molecule Caragana rhizobia exopolysaccharide (10.352kD) into the flask, then add 100 mL of 1% nitric acid solution to dissolve it, stir at room temperature for 8 hours; then weigh 1.1 g of sub Selenic acid and 0.9 g of barium chloride were added to the flask, stirred and heated at 70℃ to react for 7 hours;
[0022] 2) Add 4.32 mL of 1mol / L sulfuric acid dropwise to the above reaction solution and centrifuge at 6000 rpm for 20 minutes to remove the precipitate;
[0023] 3) Adjust the pH of the supernatant to 7 with sodium hydroxide, put it into a dialysis bag with a molecular weight cut-off of 3000Da, first dialyzed with tap water for 48 hours, then dialyzed with distilled water for 24 hours, until the dialysis is detected with 0.002mg / mL ascorbic acid solution The liquid outside the bag no longer turns red;
[0024] 4) Concentrate the dialysate under reduced pressure at 60°C and 0.08Mpa to 1 / 2 of its original volume, add 200mL 95% edible alcohol to the...
Embodiment 3
[0026] 1) Weigh 1.0 g of small molecule Rhizobium Caragana extracellular polysaccharide (30.641kD) into the flask, then add 100 mL of 2% nitric acid solution to dissolve it, stir at room temperature for 10 hours; then weigh 1.5 g of sub Selenic acid and 1.2 g of barium chloride were added to the flask, stirred and heated at 80°C for 6 hours;
[0027] 2) Add 5.76 mL of 1mol / L sulfuric acid dropwise to the above reaction solution and centrifuge at 5000 rpm for 15 minutes to remove precipitation;
[0028] 3) Adjust the pH of the supernatant to 7 with sodium hydroxide, put it into a dialysis bag with a molecular weight cut-off of 5000Da, first dialyzed with tap water for 72 hours, then dialyzed with distilled water for 36 hours, until the dialysis is detected with 0.001mg / mL ascorbic acid solution The liquid outside the bag no longer turns red;
[0029] 4) Concentrate the dialysate to 1 / 2 of its original volume under reduced pressure at 60°C and 0.08Mpa, add 150mL 95% edible alcohol to ...
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