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Primer-probe combination and kit for specific detection of Bordetella pertussis and Bordetella parapertussis

A parapertussis and kit technology, applied in the field of oligonucleotide sequence combination, can solve the problems of underestimation of the incidence of pertussis, time-consuming, poor sensitivity, etc., and achieve the effect of saving detection costs

Active Publication Date: 2016-08-03
JIANGSU UNINOVO BIOLOGICAL TECH
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Problems solved by technology

Pathogen culture is the gold standard for pertussis detection. It has strong specificity, but its sensitivity is poor. At the same time, it has the disadvantage of taking a long time and is not suitable for rapid detection in laboratories.
Serological detection of specific Bordetella pertussis and Bordetella parapertussis antibodies often requires double blood, and the detection sensitivity is low. It is mainly used for retrospective diagnosis or auxiliary diagnosis of atypical cases
[0005] The incidence of whooping cough may be underestimated due to the lack of specific laboratory tests and case diagnosis based on clinical symptoms

Method used

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  • Primer-probe combination and kit for specific detection of Bordetella pertussis and Bordetella parapertussis
  • Primer-probe combination and kit for specific detection of Bordetella pertussis and Bordetella parapertussis
  • Primer-probe combination and kit for specific detection of Bordetella pertussis and Bordetella parapertussis

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Embodiment Construction

[0027] The preferred embodiments of the present invention will be described in detail below with reference to specific examples. It should be pointed out that the embodiments listed here are only for illustrative purposes and should not be interpreted as any limitation on the scope of the present invention. The reagent kits, buffers and other reagents used therein are only the reagents specifically selected in the specific embodiment. It should be understood that those skilled in the art can select corresponding reagents from other companies as needed to achieve the purpose of the present invention.

[0028] 1. Design and synthesis of primers and TaqMan probes

[0029] Use the Blast tool to analyze the genome sequences of all Bordetella pertussis and Bordetella parapertussis in Genbank and domestic and foreign literature, and select their stable conserved regions as the detection target sequences, and target these two detections Target sequence design and synthesis of multiple set...

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Abstract

The invention relates to an oligonucleotide sequence combination for specifically detecting the presence of Bordetella pertussis and Bordetella parapertussis nucleic acids in a sample by using fluorescent PCR technology, and a kit comprising the combination. The kit can sensitively detect and identify the Bordetella pertussis nucleic acid and Bordetella parapertussis nucleic acid present in the sample, and the lower limit of detection is 20 copies per reaction system. It has great potential in disease monitoring, clinical diagnosis and other fields. important application value.

Description

Technical field [0001] The invention relates to an oligonucleotide sequence combination for specifically detecting the presence of Bordetella pertussis and Bordetella parapertussis nucleic acids in a sample by using a fluorescent PCR technology, and a kit comprising the combination. technical background [0002] Whooping cough is an acute respiratory infection caused by Bordetella pertussis. Its clinical features are paroxysmal spasmodic cough accompanied by a deep "cock crow"-like inspiratory roar. If it is not treated promptly and effectively, the course of the disease can reach several months. The disease is very contagious. The younger the child is, the more severe the condition, and death due to concurrent pneumonia and encephalopathy. [0003] Patients with whooping cough is the only source of infection. They are infectious from 1 to 2 days at the end of the incubation period to 6 weeks after the onset of illness, with the most infectious in the first 1 to 3 weeks. When a p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12R1/01
Inventor 文锋其他发明人请求不公开姓名
Owner JIANGSU UNINOVO BIOLOGICAL TECH
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