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Adaptive bacterium counting method

A bacterial counting and self-adaptive technology, applied in counting objects, counting mechanisms/items, and randomly distributed items, etc., can solve the problems of high operation skills, cumbersome operation process, high labor intensity, etc., and meet the operation skills requirements. The effect of reducing, simple operation and low labor intensity

Active Publication Date: 2012-08-15
上海禹视科技有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide an adaptive bacteria counting method to solve the technical problems of low accuracy, cumbersome operation process, high operation skill requirements and high labor intensity in the bacteria counting method in the prior art

Method used

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Embodiment Construction

[0030] Below in conjunction with the accompanying drawings, the embodiments of the present invention are described in detail: the present embodiment is implemented on the premise of the technical solution of the present invention, and provides detailed embodiments and specific operation processes, but the protection scope of the present invention is not limited to the following described embodiment.

[0031] This embodiment is described by taking the statistical counting of micrographs based on a circular petri dish of cocci as an example, and the input image is a micrograph image of cocci in a circular petri dish, such as figure 2 shown.

[0032] see figure 1 , an adaptive bacterial enumeration method comprising the following steps:

[0033] S11: Analyze the shape of the entire picture of the bacteria image obtained by the high-resolution CCD camera, identify the species, identify whether it belongs to a paper medium or a circular plate medium, and automatically extract th...

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Abstract

The invention discloses an adaptive bacterium counting method which comprises the following steps of: firstly, analyzing the form of the entire picture of the bacterium image obtained by a high-resolution CCD (charge coupled device) camera, identifying the type to determine whether the bacterium belongs to a paper culture medium or a round plate culture medium, and automatically extracting the identification area; secondly, performing HSV analysis of color space for the identification area, and automatically judging the subtype of the bacterium; then, adaptively identifying the number and shape features of the bacterium without manual intervention or manual parameter setting to finish the counting of the bacterium and the statistics of the related features; and finally, performing statistics to obtain the dimensional histogram of the bacterium according to the analysis and identification results. The method disclosed by the invention has the advantages of high accuracy and low labor intensity and is simple to operate.

Description

technical field [0001] The invention relates to a bacteria counting method, in particular to an adaptive bacteria counting method without manual intervention. Background technique [0002] At present, the widely used bacterial count method is the binary segmentation bacterial count method, which acquires bacterial images through a high-resolution CCD camera, and then performs the following series of operations on the images: first, manually identify regions for the culture medium image Extraction, then grayscale the image, and then binarize the grayscale image. In the binarization process, thresholds such as the gradient value, grayscale value, and RGB color flux of the image can be used to correct the binarization. , to obtain an ideal binarized black and white image as the input for subsequent connected region segmentation. Finally, the connected area is found for the black and white image after binarization, the connected area is segmented, and the number of bacteria is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06M11/00G06K9/46
Inventor 黄海清戚进谷朝臣胡洁
Owner 上海禹视科技有限公司
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