Preparation method for producing pigment by means of fermenting leccinum scabrum

A technology of boletus and wart stalk, which is applied in the field of microbial engineering and achieves the effects of short production cycle, wide source of raw materials and simple cultivation equipment

Inactive Publication Date: 2012-09-12
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The liquid cultured mycelium of Boletus brown verrucosus is yellow, and the yellow pigment produced by the fermentation of this fungus can be widely used in food additives, industrial printing and dyeing, biopharmaceuticals and other fields, but there is no pigment produced by the fermentation of Boletus brown verrucosus Related reports

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Preparation of boletus brown verrucosus liquid culture medium:

[0018] Take 180g of potatoes, 20g of starch, 2g of beef extract, KH 2 PO 4 0.4 g, K 2 HPO 4 0.1 g, MgSO 4 ·7H 2 O0.6 g, first wash the potatoes, peel, weigh, slice, add water and boil for 30 minutes, filter to get the filtrate, add starch, beef extract, KH to the filtrate 2 PO 4 、K 2 HPO 4 and MgSO 4 ·7H 2 O, mix evenly to 1000mL, sterilize at 121°C for 25min, and cool to obtain a liquid culture medium;

[0019] (2) Liquid fermentation of Boletus brown verrucosus: Inoculate the strain of Boletus brown verrucosus on a comprehensive PDA plate medium, culture at 26°C for 5 days to obtain the mycelia of Boletus brown verrucosus; Inoculate into liquid medium, the inoculation ratio is to add 5 pieces of mycelium with a diameter of 10mm per 100 mL of liquid medium, 26 ° C, 130r / min, dark shaking culture for 3 days, to obtain liquid seeds; the volume ratio of 8% liquid Seeds were inoculated into...

Embodiment 2

[0023] (1) Preparation of boletus brown verrucosus liquid culture medium:

[0024] Take 2000g of potatoes, 150g of starch, 10g of beef extract, KH 2 PO 4 5 g, K 2 HPO 4 2 g, MgSO 4 ·7H 2 O4 g, first wash the potatoes, peel, weigh, slice, add water and boil for 30 minutes, filter to get the filtrate, add starch, beef extract, KH to the filtrate 2 PO 4 、K 2 HPO 4 and MgSO 4 ·7H 2 O, mix evenly to 10L, sterilize at 121°C for 25min, and cool to obtain a liquid culture medium;

[0025] (2) Liquid fermentation of Boletus brown verrucosus: inoculate the strains of Boletus brown verrucosus on the comprehensive PDA plate medium, culture at 27°C for 5 days to obtain the mycelia of Boletus brown verrucosus; Inoculate into liquid medium, the inoculation ratio is to add 5 pieces of mycelium with a diameter of 10mm per 100 mL of liquid medium, 27 ° C, 130r / min, dark shaking culture for 3 days, to obtain liquid seeds; the volume ratio of 10% liquid Seeds were inoculated into l...

Embodiment 3

[0029] (1) Preparation of boletus brown verrucosus liquid culture medium:

[0030] Take 1500g of potatoes, 180g of starch, 15g of beef extract, KH 2 PO 4 5 g, K 2 HPO 4 2 g, MgSO 4 ·7H2 O2 g, firstly wash, peel, weigh and slice the potatoes, add water and boil for 30 minutes, filter to get the filtrate, add starch, beef extract, KH to the filtrate 2 PO 4 、K 2 HPO 4 and MgSO 4 ·7H 2 O, mix evenly to 10L, sterilize at 121°C for 25min, and cool to obtain a liquid culture medium;

[0031] (2) Liquid fermentation of Boletus brown verrucosus: inoculate the strains of Boletus brown verrucosus on the comprehensive PDA plate medium, culture at 25°C for 7 days to obtain the mycelia of Boletus brown verrucosus; Inoculate into liquid medium, the inoculation ratio is to add 5 pieces of mycelium with a diameter of 10mm per 100 mL of liquid medium, 25 ° C, 130r / min, dark shaking culture for 3 days, to obtain liquid seeds; the volume ratio of 9% liquid Seeds were inoculated into...

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Abstract

A preparation method for producing pigment by means of fermenting leccinum scabrum includes steps of preparing a liquid culture medium; fermenting the leccinum scabrum to obtain mycelium powder; ultrasonically extracting leccinum scabrum mycelium powder pigment in an auxiliary manner; and the like. In the method, liquid culture mycelium is fast in breeding speed, pigment yield is high, nutritional values are high, culture equipment is simple, sources of raw materials are wide, prices are cheap, large-scale production can be realized, ultrasonic extraction production period is short, an operation process is controlled easily, extraction efficiency is high, energy conservation and environmental protection are realized, an economic benefit is high, and industrial popularization is facilitated.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a preparation method for fermenting and producing pigment by boletus spp. Background technique [0002] Pigments are colorants, which are widely used in food, daily chemical, medicine, printing and dyeing industries. According to different sources, pigments can be divided into two types: natural pigments and synthetic pigments. Because synthetic pigments have certain toxicity, people prefer natural pigments. There are three main sources of natural pigments, plants, animals, and microorganisms. Through large-scale cultivation of microorganisms, fermentation and production of pigments are not limited by resources, environment and space. It is an effective way to obtain pigments with high efficiency and low cost. Fusaractin, etc. my country is rich in macrofungi resources, especially some macrofungi have high pigment content and have both nutritional and pharmacol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 刘朝贵方琼陈仁玉
Owner SOUTHWEST UNIV
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