Mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample and establishing method and application thereof

Abstract

The invention discloses a mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample and an establishing method and application thereof. The mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample is prepared by the steps of bacterial strain cultivation and identification, preparation of a nucleic acid standard sample, drying of the nucleic acid standard sample, inspection of uniformity and stability, qualitative verification, valuing of the nucleic acid standard sample and the like. The situation of shortage in nucleic acid standard samples of pathogenic bacteria in domestic foods is solved. The mononuclear cell proliferation listeria monocytogenes nucleic acid standard sample, the establishing method and the application have very important practical significance on solving of a preparative technology of the nucleic acid standard samples of the pathogenic bacteria in the foods and a stability guarantee technology, active implementation of development of molecular biological test standard samples of the pathogenic bacteria in the domestic foods, filling of the blank in the measurement field and the implementation of molecular biological detection work of the pathogenic bacteria in the foods. The nucleic acid standard samples of the pathogenic bacteria in the foods have wide market and large economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to the preparation of a Listeria monocytogenes nucleic acid standard sample, its establishment method and its application in the detection of Listeria monocytogenes. Background technique [0002] Food safety is a major public health issue and an important factor restricting my country's economic development. Foodborne diseases caused by microbial contamination are the most prominent problems in my country's food safety. In recent years, with the adjustment of food industry structure, the rapid development of economic globalization and international trade, the prevention and control of foodborne diseases in my country is facing a series of new problems and challenges. [0003] The prevention and control of foodborne diseases caused by microorganisms in food has attracted the attention of countries all over the world. The detection technology of microorganisms in f...

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Problems solved by technology

[0004] Due to the particularity of bacteria in food, it is difficult for people to obtain positive samples for bacterial molecular biology detection. In addition, the research on molecular biology detection technology of pathogenic bacteria in food has just started, while the research work on supporting standard samples started late. Preparation The technology is complicated, and there are many difficulties in the evaluation of sample value and uncertainty, so the standard samples (quantitative and qualitative tests) of food pathogenic bacteria nucleic acid at home and abroad are basically blank

At present, there are no nucleic acid standard samples suitable for the molecular biology detection of pathogenic bacteria in food at home and abroad. There are only positive controls equipped in a complete set of molecular biology detection kits. Meeting the needs of inspection and quarantine work in a timely manner

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