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Precise localization method for analysis of Elispot by using image identification

An image recognition and precise positioning technology, which is applied in image analysis, image data processing, character and pattern recognition, etc., can solve problems such as complexity, image depth and uniformity, etc., to reduce requirements and solve the problem of precise cutting Effect

Inactive Publication Date: 2012-09-26
上海铭源数康生物芯片有限公司 +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, the microplate image of Elispot is the image after biochemical reaction, the depth of the image is uncertain, and the uniformity is also poor. It is an image similar to a natural scene, and image analysis is a relatively complicated problem.

Method used

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  • Precise localization method for analysis of Elispot by using image identification
  • Precise localization method for analysis of Elispot by using image identification
  • Precise localization method for analysis of Elispot by using image identification

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Embodiment Construction

[0039] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0040] like figure 1 Shown, the application image recognition of the present invention is to the automatic accurate positioning method of Elispot speckle analysis, mainly comprises the following steps:

[0041] 1) Obtain a grayscale image of the original microplate

[0042] see figure 2 and image 3, the captured image is generally a color image. When performing Elispot spot analysis, the color information is not needed, and the brightness information contained in the image is mainly used, so this step is to convert the color image into a grayscale image; for direct For grayscale images captured by a black and white camera, this step is not required. A color image can be converted to a grayscale image using the following formula:

[0043] Y=...

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Abstract

The invention discloses a precise localization method for analysis of Elispot by using image identification. The method mainly comprises the steps of transforming grey level value of an original micro-well plate single-pore coloured image; detecting the flange of the grey level image; searching for pore bottom circle of the flange detection image; carrying out pore bottom trimming for an original whole origin image. According to the method disclosed by the invention, the image characteristic of an Elispot analysis instrument is combined, the pore bottom circumference searching is conducted based on the micro-well plate based on flange detected image, the problem on precise trimming of the micro-well plate pore bottom image is solved, and the demands on positioning of a motor and manufacture precision of the micro-well plates are lowered. The method of the invention can be used for image analysis of an Elispot reader and also for similar target area positioning based on the micro-well plate image analysis.

Description

technical field [0001] The invention relates to the field of in vitro diagnostic instruments, in particular to a method for precisely positioning the hole bottom image of a microplate in a spot reader. Background technique [0002] ELISPOT technology is enzyme-linked immunospot detection technology, which is an immunological detection technology. This technology combines the strengths of cell culture technology and enzyme-linked immunosorbent assay (ELISA technology), and has a series of advantages: extremely high sensitivity, can detect the secretion of cytokines at the single-cell level, and can detect live immune cells. function and state, and the operation is simple and economical, and high-throughput screening can be performed. It is the only detection technology that can detect one-per-million positive cell rate today, and its sensitivity is at least 1-2 orders of magnitude higher than ordinary ELISA technology, flow cytometry (intracellular cytokine staining) and Tet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06T7/00G06K9/32
Inventor 田涌涛吴雪琼梁艳许颖原张香琴姚涌
Owner 上海铭源数康生物芯片有限公司
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