Pretreatment method for high-efficiency enzyme hydrolysis of wood waste fiber

A waste fiber and pretreatment technology, applied in the direction of fermentation, etc., can solve the problems of polluting the environment, destroying the ecology, and the waste fiber cannot be fully and effectively recycled, and achieve the goals of reducing volume consumption, saving water consumption, and high enzymatic hydrolysis effect Effect

Inactive Publication Date: 2013-12-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The pulp and paper industry produces a large amount of waste fibers, and manufacturers often landfill or incinerate these waste fibers. The waste fibers not only cannot be fully and effectively recycled, but also seriously damage the ecology and pollute the environment.

Method used

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  • Pretreatment method for high-efficiency enzyme hydrolysis of wood waste fiber
  • Pretreatment method for high-efficiency enzyme hydrolysis of wood waste fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) Preprocessing

[0027] Weigh 30 g of wood waste fiber with an absolute dry weight and place it in an autoclave, add 3.893 g of ferric chloride, and add an appropriate amount of water to adjust the solid-liquid ratio to 1:8 (unit: g / mL), stir and mix After uniformity, start to heat up, and keep warm for 20 min after reaching the highest temperature of 180 °C. Immediately after the pretreatment, steam is released, unloaded and cooled for solid-liquid separation, and the pretreated fibers are washed with water until neutral for biological enzyme hydrolysis.

[0028] 2) Cellulase hydrolysis rate of pretreated raw materials

[0029] Weigh 1 g of pretreated fibers with a dry weight in a 100 mL Erlenmeyer flask, add acetic acid-sodium acetate buffer solution (pH = 4.8), and mix in a constant temperature shaker at a temperature of 50 °C and a speed of 150 rpm 15 min to wet and disperse the fibers. Add 20 FPU of cellulase and 25 CBU of β-glucosidase and add water so that t...

Embodiment 2

[0031] 1) Preprocessing

[0032] Weigh 30 g of wood waste fiber with an absolute dry weight and place it in an autoclave, add 3.893 g of ferric chloride, and add an appropriate amount of water to adjust the solid-liquid ratio to 1:8 (unit: g / mL), stir and mix After uniformity, start to heat up, and keep warm for 0 min after reaching the highest temperature of 200 °C. Immediately after the pretreatment, steam is released, unloaded and cooled for solid-liquid separation, and the pretreated fibers are washed with water until neutral for biological enzyme hydrolysis.

[0033]2) Cellulase hydrolysis rate of pretreated raw materials

[0034] Weigh 1 g of pretreated fibers with a dry weight in a 100 mL Erlenmeyer flask, add acetic acid-sodium acetate buffer solution (pH = 4.8), and mix in a constant temperature shaker at a temperature of 50 °C and a speed of 150 rpm 15 min to wet and disperse the fibers. Add 20 FPU of cellulase and 25 CBU of β-glucosidase and add water so that the...

Embodiment 3

[0036] 1) Preprocessing

[0037] Weigh 30 g of wood waste fiber with an absolute dry weight and place it in an autoclave, add 3.893 g of ferric chloride, and add an appropriate amount of water to adjust the solid-liquid ratio to 1:8 (unit: g / mL), stir and mix After uniformity, start to heat up, and keep warm for 240 min after reaching the highest temperature of 180 °C. Immediately after the pretreatment, steam is released, unloaded and cooled for solid-liquid separation, and the pretreated fibers are washed with water until neutral for biological enzyme hydrolysis.

[0038] 2) Cellulase hydrolysis rate of pretreated raw materials

[0039] Weigh 1 g of pretreated fibers with a dry weight in a 100 mL Erlenmeyer flask, add acetic acid-sodium acetate buffer solution (pH = 4.8), and mix in a constant temperature shaker at a temperature of 50 °C and a speed of 150 rpm 15 min to wet and disperse the fibers. Add 20 FPU of cellulase and 25 CBU of β-glucosidase and add water so that ...

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Abstract

The invention relates to a pretreatment method for high-efficiency enzyme hydrolysis of wood waste fiber. According to the pretreatment method, the wood waste fiber is pretreated by using mixed solution of metal chloride solution and a surfactant, the pretreated raw material is subjected to cellulase hydrolytic saccharification; and reducing sugar obtained by hydrolysis can be used for producing a biofuel by fermentation. Glucose in enzymatic hydrolysate is determined by using a glucose oxidase-peroxidase method, and total glucan in the raw material is determined by using acid process hydrolysis, so that the hydrolysis rate of the glucose in the raw material is calculated. After the wood waste fiber is pretreated under the pretreatment conditions that the mass ratio of metal chloride to the surfactant is 2.4-4.8 (w / w), the temperature is 160-200 DEG C, the heat preservation time is 0-240 minutes and the liquid-solid ratio is 8:1, hydrolysis is performed by using a complex enzyme of cellulase and beta-glucosaccharase to produce the glucose, and the final hydrolysis rate reaches 60-91 percent.

Description

technical field [0001] The invention relates to the technical field of processing waste wood fibers, in particular to a pretreatment method for high-efficiency enzymatic hydrolysis of waste wood fibers. Background technique [0002] Energy crisis, environmental pollution and ecological damage seriously restrict the sustainable development of human society. Lignocellulosic fiber is a renewable resource with large reserves, and is considered to be an ideal substitute for petrochemical resources. In recent years, the biorefining of lignocellulosic fibers, especially the preparation of fuel ethanol from lignocellulosic fibers, has become a research hotspot at home and abroad. The key step in the production of ethanol from wood fiber is the pretreatment of wood fiber, which affects a series of subsequent processes. There are many pretreatment methods for wood fiber, such as caustic soda method, dilute acid method, hot water / steam explosion method, ammonia method and organic sol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 付时雨陈理恒刘浩
Owner SOUTH CHINA UNIV OF TECH
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