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Application of naphthylethylenediamine inorganic acid salt or Naphthylethylenediamine organic acid salt as matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry)

A technology of naphthalene ethylene diamine inorganic acid salt and naphthalene ethylene diamine hydrochloride, which is applied in the field of mass spectrometry detection, can solve the problems of long time consumption, decreased analysis effect, poor repeated use effect, etc., and achieves reduction requirements and sample compatibility. Good results

Active Publication Date: 2014-05-14
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When desorption / ionization occurs on the porous silicon surface, the surface of the silicon crystal must first be etched to produce a nanostructured surface. Although peptides and antiviral drugs can be directly analyzed on such a surface, the repeated use of this surface is not effective. OK, so repeated treatments on the same nanostructured surface are required to get a reusable surface
In addition, it takes a long time to chemically modify the surface of porous silicon, and the analysis effect will be greatly reduced when the modified porous silicon is reused.

Method used

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  • Application of naphthylethylenediamine inorganic acid salt or Naphthylethylenediamine organic acid salt as matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry)
  • Application of naphthylethylenediamine inorganic acid salt or Naphthylethylenediamine organic acid salt as matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry)
  • Application of naphthylethylenediamine inorganic acid salt or Naphthylethylenediamine organic acid salt as matrix in MALDI MS (matrix-assisted laser desorption/ionization mass spectrometry)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, naphthalene ethylenediamine hydrochloride identification distinguishes different microorganisms (bacteria)

[0031] The cultured Mycobacterium smegmatis and Escherichia coli and their different subspecies and mutant strains were collected after growing to greater than OD0.6, washed with phosphate buffered saline and stored at -20°C or directly used for further processing; phosphate buffered saline The suspended bacteria were replaced with deionized water, centrifuged at 5000rpm to retain the precipitate, 3 times the volume of 80% ethanol aqueous solution (volume percentage content) was added to the precipitate, the suspension was allowed to stand for 15min, and the supernatant was taken after centrifugation at 5000rpm. Centrifuge directly to take an appropriate amount of sample for mass spectrometry analysis.

[0032] Get sample and matrix solution (the ethanol aqueous solution of naphthalene ethylenediamine hydrochloride (wherein, the volume ratio of etha...

Embodiment 2

[0034] Example 2. Mass Spectrometry Imaging of Mouse Brain Tissue Sections

[0035] After the mouse brain tissue was quick-frozen, without fixing and embedding, the standard frozen section tissue processing method was used to cut a 10um thick section and place it on an ITO conductive glass slide to spray a matrix solution (an ethanol aqueous solution of naphthalene ethylenediamine hydrochloride, wherein ethanol The volume ratio with water is 1: 2, and the mass volume concentration of naphthalene ethylenediamine hydrochloride is 10mg / ml); The spraying of matrix is ​​done automatically, according to the default method of specific imaging mass spectrometry; Analyze according to the standard mass spectrometry imaging method, mass spectrometry : Bruker Ultraflextreme TOF; 100um resolution (limit resolution 10um); 300 spectra per pixel; laser wavelength 355nm, pulse frequency 1000Hz; 38% intensity; m / z 100-1000; negative ion mode.

[0036] image 3 It is the different distribution ...

Embodiment 3

[0037] Embodiment 3, the detection of metal ion under serum background

[0038] Both naphthaleneethylenediamine hydrochloride and naphthaleneethylenediamine nitrate can be used for the detection of metal ions. In the MALDI ionization mode, negative ions (Cl - or NO 3 - ) is combined with positively charged metal ions, and an additional negative ion is combined to make the whole negatively charged, so that it can be separated and detected.

[0039] Get 100ug / L standard to add the serum of lead acetate standard sample and matrix solution (the ethanol aqueous solution of naphthalene ethylenediamine nitrate, wherein, the volume ratio of ethanol and water is 1: 2, and the mass volume concentration of naphthalene ethylenediamine nitrate is 10mg / ml ) at a volume ratio of 1:1 (the molar ratio of naphthalene ethylenediamine nitrate to lead acetate is 10:1), add 1 ul sample to the MALDI target plate, and fully dry. Into mass spectrometry, mass spectrometry conditions are: voltage: a...

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Abstract

The invention discloses application of naphthylethylenediamine inorganic acid salt or naphthylethylenediamine organic acid salt as a matrix in MALDI MS (matrix-assisted laser desorption / ionization mass spectrometry). In the application, the MALDI MS is MALDI-TOF MS (matrix-assisted laser desorption / ionization - time-of-flight mass spectrometry); the naphthylethylenediamine inorganic acid salt is naphthylethylenediamine hydrochloride, naphthylethylenediamine nitrate, naphthylethylenediamine phosphate or naphthylethylenediamine sulfate; and the naphthylethylenediamine organic acid salt is naphthylethylenediamine trifluoroacetate, naphthylethylenediamine acetate, naphthylethylenediamine formate, naphthylethylenediamine citrate or naphthylethylenediamine oxalate. The invention technically overcomes the defect that small-molecule samples cannot be effectively analyzed because serious matrix background interference is apt to be caused in a low molecular weight zone when organic small-molecule matrices are commonly used. The matrix used by the invention is not required to be added with ionizing reagent and the requirements on sample treatment are reduced; and since background interference hardly exists within a testing range (m / z: 0-1000), kinds of complex mixed systems can also be analyzed by using the matrix.

Description

technical field [0001] The invention relates to the application of naphthaleneethylenediamine inorganic acid salt or naphthaleneethylenediamine organic acid salt as a matrix in MALDI, and belongs to the technical field of mass spectrometry detection. Background technique [0002] Since Karas et al. and Tanaka et al. reported in 1988 that matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology can be used for effective mass spectrometry analysis of biological macromolecules, MALDI-TOF MS technology has been favored by researchers from various countries. of favor. However, since the commonly used matrices of MALDI-TOF MS technology are small molecular organic compounds, such as α-cyano-4-hydroxycinnamic acid (CCA), 2,5-dihydroxybenzoic acid (DHB), sinapinic acid (SA), 3-Hydroxy-2-pyridinecarboxylic acid (3-HPA), anthracenol (DI) and 3-aminoquinoline (3-AQ), etc. In the analysis process, because the above-mentioned matrix molecul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/64
Inventor 聂宗秀王佳宁陈芮侯剑陈素明
Owner INST OF CHEM CHINESE ACAD OF SCI
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