Unlock instant, AI-driven research and patent intelligence for your innovation.
Upland cotton GhLFY protein and coding gene and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology that encodes genes and genes, which is applied in the fields of application, genetic engineering, plant genetic improvement, etc., and can solve the problems of insufficient prematurity of short-season cotton
Active Publication Date: 2015-04-01
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
View PDF6 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, the current short-season cotton has the problem of insufficient early maturity
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0053] Embodiment 1, the cloning of cotton gene GhLFY
[0054] 1. Cloning of cotton gene GHLFY
[0055] 1. Extraction of RNA
[0056] According to the CTAB method, RNA extraction steps:
[0057] (1) Place a 50ml centrifuge tube on the centrifuge tube rack, add 15ml extract solution to the centrifuge tube, add 300ul β-mercaptoethanol, and preheat the water bath at 65°C for 10 minutes;
[0058] (2) Clean and dry the mortar and pestle, pour a little alcohol into it to burn and sterilize, add liquid nitrogen to pre-cool after cooling, put 2-3g of different tissues of the early-maturing cotton variety Zhongmian 36 into the cooled mortar , quickly grind, and after grinding into a fine powder, scrape it into the preheated extract at 65°C with a pre-cooled spoon, shake vigorously, and put it in a warm bath at 65°C for 10 minutes, shaking 3-4 times during the period;
[0059] (3) Add an equal volume of chloroform: isoamyl alcohol (24:1), shake vigorously for 10 minutes, 13000 rpm, a...
Embodiment 2
[0120] Embodiment 2, the application of cotton gene GhLFY in promoting plant flowering
[0121] 1. Construction of expression vector
[0122]Using the cDNA of Zhongmian Institute 36 terminal bud as a template (or artificially synthesized sequence 1 as a template), the infusion primer (F: 5'-CACTGTTGATACATATGCTTCAAAAATGGACCCTGAGAC-3' (sequence 3); R: 5'-TGTTGATTCAGAATTCTTCAGTTCCAACTTAAACCATACA-3' ( Sequence 4), amplified to obtain a PCR product containing the complete open reading frame of the GhLFY gene, after sequencing, it is the 3rd-1300 nucleotides from the 5' end of Sequence 1 in the sequence listing;
[0123] The plant expression vector pRI101 (Takara Code: D3262) was digested with NdeI and EcoRI to obtain the vector backbone;
[0124] The above vector backbone was ligated with the above PCR product using Clotech's Infusion ligation kit.
[0125] The enzyme digestion system of pRI101 is as follows:
[0126]
[0127] The connection system of the Infusion Ligation Ki...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Login to View More
Abstract
The invention discloses upland cotton GhLFY protein and a coding gene and application thereof. The protein is protein in (a) or (b): (a) protein consisting of an amino acid sequence shown as Sequence 2 in a sequence table; and (b) plant flower-related protein derived from Sequence 2 via substitution and / or deletion and / or addition of one or a plurality of amino acid residue(s) of the amino acid sequence shown as Sequence 2 in the sequence table. Experiments show that a new gene GhLFY is discovered, the transgenic plant modified genetically by the new gene can flower in advance, seeds can also be obtained in advance, the number of rosette leaves of the plant can be reduced, and the number of cauline leaves can be increased; and thus the gene can be applied in cultivation of cotton with a short growth period, and a foundation is laid for cultivation of genetically modified plants.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a GhLFY protein of upland cotton and its coding gene and application. Background technique [0002] Cotton is one of the most important economic crops in our country and plays an important role in national production. However, the development of cotton is limited to a certain extent due to the land competition between grain and cotton. Breeding and promotion of short-season cotton varieties can alleviate the conflict between grain and cotton land, and is an effective way to achieve double harvests of grain and cotton. However, the current short-season cotton has the problem of insufficient prematurity. [0003] As one of the important traits of cotton, early maturity has become an important breeding goal of upland cotton. Studies have shown that flowering sooner or later is closely related to the early maturity of cotton. Therefore, the flowering-related genes were cloned, and the...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More