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Selenylation modification method for improving immunity-enhancing activity of angelica polysaccharide

A technology of immune enhancement and modification method, which is applied in the field of structural modification of polysaccharides in traditional Chinese medicine, which can solve the problems of low biological activity of polysaccharides and achieve the effect of enhancing immune activity

Active Publication Date: 2012-10-10
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0006] Technical problem The present invention aims at the problem of low biological activity of polysaccharides in traditional Chinese medicine, and provides a selenization modification method through the immune enhancement activity of angelica polysaccharides, so as to achieve the purpose of significantly improving the immune-enhancing activity of angelica polysaccharides

Method used

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  • Selenylation modification method for improving immunity-enhancing activity of angelica polysaccharide
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  • Selenylation modification method for improving immunity-enhancing activity of angelica polysaccharide

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Embodiment Construction

[0015] 1. Extraction of polysaccharides from Angelica sinensis

[0016] Decoction with water - alcohol precipitation method. Take 1000g of angelica decoction pieces, crush them into 0.3-1cm 3 Add 2000mL of 95% ethanol to soak for 12h, reflux twice in 80℃ water bath, each time for 1h, let the drug dry for 12h, add 20 times the amount of water to decoct twice, each time for 30min, combine the filtrate, concentrate to 1000mL, and centrifuge at 2500rpm After 20 minutes, the precipitate was discarded, and 95% ethanol was added to make the alcohol content reach 90%, and the mixture was allowed to stand at 4°C for 12 hours, the ethanol was recovered, centrifuged, and the precipitate was vacuum-dried at 60°C to obtain crude angelica polysaccharide.

[0017] 2. Purification of Angelica polysaccharide

[0018] The crude angelica polysaccharide was deproteinized by Sevage method, dried in vacuum, and dissolved in distilled water to 50 mg·mL -1 solution, put it on a Sephadex G-200 (2cm...

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Abstract

The invention discloses a selenylation modification method for improving immunity-enhancing activity of angelica polysaccharide and belongs to the technical field of structural modification of Chinese herbal medicinal polysaccharide. The angelica polysaccharide is extracted and purified; the selenium modification is performed on the angelica polysaccharide by a nitric acid-sodium selenite method; with the immunity-enhancing activity of a product as an index, an L9 (34) orthogonal experiment is performed to optimize three factors, namely the using amount of the sodium selenite, the reaction temperature and the reaction time, so as to determine the optimal modification conditions that the using amount of the sodium selenite is 200 mg (500 mg of the angelica polysaccharide), the reaction temperature is 70 DEG C and the reaction time is 6 hours. The immunity-enhancing activity of the angelica polysaccharide can be significantly improved.

Description

1. Technical field [0001] The invention discloses a selenization modification method of angelica polysaccharides, which belongs to the technical field of structure transformation of traditional Chinese medicine polysaccharides. 2. Background technology [0002] Polysaccharides widely exist in plants, microorganisms, algae and animals, and are one of the four basic substances that constitute life. With the rapid development of chemistry and biology and the improvement of separation technology, the biological functions of polysaccharides, especially as living substances to participate in the full time and space functions of life, have broken through the traditional concept of polysaccharides as supporting tissues and energy sources. It has a wider range of biological effects, such as enhancing immunity, anti-tumor, anti-inflammatory, anti-bacterial, anti-virus, anti-oxidation, anti-mutation, anti-aging, lowering blood sugar, lowering blood fat, etc., and has the advantages of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04
Inventor 胡元亮秦韬王德云姚万玲刘家国
Owner NANJING AGRICULTURAL UNIVERSITY