Selenized garlic polysaccharide with immunological enhancement effect and preparation method of selenized garlic polysaccharide
A garlic polysaccharide, immunity enhancement technology, applied in the direction of instruments, analytical materials, allergic diseases, etc., can solve the problems of low immunity, vaccine immunization failure, etc., and achieve the effects of increasing polysaccharide content, improving stability, and shortening the preparation period
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Embodiment 1
[0036] refer to figure 1 , a kind of selenized garlic polysaccharide with immunoenhancing effect and preparation method thereof, comprises the following steps:
[0037] S1: Put 500mg of garlic polysaccharide into a 250ml triangular cup, slowly add 0.5% HNO350mL dropwise, and stir while adding to fully dissolve;
[0038] S2: Add three different milliliters of sodium selenite to the solution configured in S1, respectively 200mg, 300mg, and 400mg, and stir evenly to make full contact, and place it in a constant temperature water tank for reaction. The reaction temperature is 50°C, and the reaction time is respectively After 6h, 8h, and 10h of reaction, cool to room temperature to obtain three kinds of selenized garlic polysaccharides, namely sGPS1, sGPS2, and sGPS3, and weigh them to calculate the yield of selenized garlic polysaccharides, which are 30.33%, 31.33%, and 26.28%, respectively;
[0039]S3: Take the sGPS1, sGPS2, and sGPS3 configured in S2, and use the phenol-sulfuri...
Embodiment 2
[0042] refer to figure 1 , a kind of selenized garlic polysaccharide with immunoenhancing effect and preparation method thereof, comprises the following steps:
[0043] S1: Put 500mg of garlic polysaccharide into a 250ml triangular cup, slowly add 0.5% HNO350mL dropwise, and stir while adding to fully dissolve;
[0044] S2: Add three different milliliters of sodium selenite to the solution configured in S1, respectively 200mg, 300mg, and 400mg, and stir evenly to make full contact, and place it in a constant temperature water tank for reaction. The reaction temperature is 70°C, and the reaction time is respectively After 8h, 10h, and 6h, the reaction was cooled to room temperature, and three kinds of selenized garlic polysaccharides were obtained, namely sGPS4, sGPS5, and sGPS6. They were weighed, and the yields of selenized garlic polysaccharides were calculated, which were 28.80%, 27.22%, and 30.56%, respectively;
[0045] S3: Take the sGPS4, sGPS5, and sGPS6 configured in ...
Embodiment 3
[0048] refer to figure 1 , a kind of selenized garlic polysaccharide with immunoenhancing effect and preparation method thereof, comprises the following steps:
[0049] S1: Put 500mg of garlic polysaccharide into a 250ml triangular cup, slowly add 0.5% HNO350mL dropwise, and stir while adding to fully dissolve;
[0050] S2: Add three different milliliters of sodium selenite to the solution configured in S1, respectively 200mg, 300mg, and 400mg, and stir evenly to make full contact, and place it in a constant temperature water tank for reaction. The reaction temperature is 90°C, and the reaction time is respectively After 10h, 6h, and 8h, the reaction was cooled to room temperature, and three kinds of selenized garlic polysaccharides were obtained, namely sGPS7, sGPS8, and sGPS9. They were weighed, and the yields of selenized garlic polysaccharides were calculated, which were 15.30%, 22.36%, and 16.86%, respectively;
[0051] S3: Take the sGPS7, sGPS8, and sGPS9 configured in ...
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