Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf

Abstract

The invention provides a method for producing a film of leaf epidermis of a stomatal pit of a sweetscented oleander leaf. The method comprises the steps of drawing materials and producing the film and further comprises the steps of the crosscutting film production of the sweetscented oleander leaf and the film production of the leaf epidermis at the bottom of the stomatal pit. In the step of the film production of the leaf epidermis at the bottom of the stomatal pit, mainly a micromanipulation method is utilized; a needle point with an inclined plane structure is used as dissection equipment; and after upper epidermis and mesophyll tissues on a superficial layer are picked and scrapped and when leaf veins and mesophyll tissues around the stomatal pit are picked and scrapped by the needle point, the mesophyll tissues attached onto the stomatal pit epidermis are scrapped by utilizing a cutting surface of the needle point. According to the invention, the film production of the leaf epidermis of the stomatal pit of the sweetscented oleander leaf is implemented by adopting the unarmed crosscutting film production of the leaf and the micromanipulation method; the method is simple to operate; the film production process is simplified; the film production time is shortened; and the shapes of leaf epidermis cells on the inner side walls and at the bottom of the stomatal pit of the sweetscented oleander leaf and the composition of a stomatal apparatus are observed.

Description

technical field [0001] Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for preparing sheets of stomatal fossa leaf epidermis of oleander leaves. Background technique: [0002] Oleander ( Nerium indicum Mill.) is a common plant in the family Apocynaceae. In various textbooks and experimental textbooks such as "Botany", "Plant Biology" and "Plant Anatomy", almost all of them are planted with oleander. The leaf structure is described as a typical leaf of xerophytes. [0003] The upper and lower epidermis of oleander leaves are both stratified epidermis, and the stomata only grow in the stomatal nests sunken in the mesophyll tissue of the lower epidermis, and only the openings of the stomatal nests can be seen on the surface of the lower epidermis, and the stomata and their surrounding epidermis. Usually only the longitudinal section structure of the stomatal nest and stomata can be observed through the...

AI Technical Summary

Problems solved by technology

[0003] The upper and lower epidermis of oleander leaves are both stratified epidermis, and the stomata only grow in the stomata nests sunken in the mesophyll tissue of the lower epidermis. Only the opening of the stomatal fossa can be seen, the stomata and surrounding epidermis cannot be observed

Usually, only the longitudinal section structure of the stomatal nest and stomata can be observed through the transverse section of oleander leaves, but the cell shape and stomatal structure of the leaf epidermis in the stomatal nest cannot be observed

Due to the special structure of the stomata of oleander leaves, all kinds of leaf epidermis preparation methods cannot obtain the leaf epidermis of its stomata. a puzzle on

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