Heatproof lyophilized protective agent for live vaccine JXA1-R strain for highly pathogenic porcine reproductive and respiratory syndrome and method for preparing the same

Abstract

The invention relates to a heatproof lyophilized protective agent for a live vaccine JXA1-R strain for highly pathogenic porcine reproductive and respiratory syndrome and a method for preparing the same. The heatproof lyophilized protective agent for the live vaccine JXA1-R strain for the highly pathogenic porcine reproductive and respiratory syndrome in the invention is composed of the followingcomponents, by weigh: 0.8 to 1.2% of gelatin, 4 to 6% of soybean peptone, 0.5 to 1.5% of glycine, 4 to 6% of trehalose, 1 to 2% of polyvinyl pyrrolidone, 0.5 to 1% of mannitol, and the balance being water for injection; The heatproof lyophilized protective agent is prepared through the steps of high-temperature sterilization, microporous membrane filtration degerming and mixing. The heatproof lyophilized protective agent for the live vaccine JXA1-R strain for the highly pathogenic porcine reproductive and respiratory syndrome in the invention reduces damages to virus activity, the damages being caused by physicochemical factors in lyophilization of the vaccine, and enables virus loss rate to be lower before and after lyophilization with the virus loss rate being no more than 0.2 titer; further, the heatproof lyophilized protective agent satisfies the requirements for low-temperature refrigerated transport of the vaccine and effectively solves the problems such as inconvenient storage, high cost of the vaccine and the like.

Description

[0001] technical field [0002] The invention belongs to the technical field of veterinary biological products, and relates to a freeze-drying technology for producing live virus vaccines for animals, in particular to a heat-resistant freeze-drying protectant for highly pathogenic porcine reproductive and respiratory syndrome live vaccines and a preparation method thereof. [0003] Background technique [0004] Among the veterinary vaccines in my country, freeze-dried live vaccines account for a considerable proportion. According to different production processes and storage conditions, they can be divided into conventional protective agent live vaccines and heat-resistant protective agent live vaccines. At present, most domestic products are Conventional protective agents live vaccines. [0005] The main components of conventional protective agents are skimmed milk, sucrose, gelatin, etc. The formula is simple, the preparation is easy, and the solubility is good, but the th...

AI Technical Summary

Problems solved by technology

[0010] In order to overcome the defects of the prior art, the object of the present invention is to provide a heat-resistant freeze-drying protectant for highly pathogenic porcine reproductive and respiratory syndrome live vaccine JXA1-R strain, which has good protection performance and can make