Method for extracting sodium copper chlorophyllin from wild deyeuxia angustifolia forage grass

A technology of copper chlorophyll sodium salt and chlorophyll, which is applied in the preparation methods of peptides, chemical instruments and methods, plant peptides, etc., to achieve the effects of easy operation, simple method and low production cost.

Active Publication Date: 2012-10-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic extraction of sodium copper chlorophyllin mostly uses silkworm dung, bamboo leaves, and plantain leaves a

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0024] Example 1: A two-step method for extracting chlorophyll and grass protein. Paste chlorophyll is saponified with lye, and copper is replaced with a new method of sodium copper chlorophyllin. The steps are as follows;

[0025] (1) 1000g of fresh small-leaf camphor grass cut 3cm, 6 times 80% ethanol extraction, add to a mortar to grind into a homogenate, stand still, filter to obtain the filtrate, rinse the mortar with ethanol;

[0026] (2) Pass the filter residue through a squeezer to obtain the remaining fresh juice, filter, combine all the filtrates and add gasoline, stir them thoroughly, put them in a separatory funnel, and let them stand for more than 2 hours. The liquid is divided into three layers.

[0027] (3) The upper layer is the oil layer, which is dark green, the lower layer is yellow liquid, called the water layer, the middle layer is suspended above the water layer, and the lower layer is called the gray sedimentary layer. Filter the water layer, gray precipitatio...

Example Embodiment

[0033] Implementation case 2:

[0034] (1) 1000g of fresh small-leaf camphor grass, cut 4cm, 7 times of 85% ethanol extraction, add to a mortar to grind into a homogenate, stand still, filter to obtain the filtrate, rinse the mortar with ethanol;

[0035] (2) Pass the filter residue through a squeezer to obtain the remaining fresh juice, filter, combine all the filtrates and add gasoline, stir them thoroughly, put them in a separatory funnel, and let them stand for more than 3 hours. The liquid is divided into three layers.

[0036] (3) The upper layer is the oil layer, which is dark green, the lower layer is a yellow liquid called the water layer, the middle layer is suspended above the water layer, and the lower layer is called the gray sedimentary layer. Filter the water layer, gray precipitation layer, and oil layer separately, combine the filter residues, wash with distilled water and drain, and dry at 90°C to obtain 83.7 g of grass protein; recover ethanol and gasoline from the...

Example Embodiment

[0042] Implementation case 3:

[0043] (1) 1000g of fresh small-leaf camphor grass, cut 5cm, 8 times of 90% ethanol extraction, add to a mortar to grind into a homogenate, stand still, filter the filtrate, rinse the mortar with ethanol;

[0044] (2) Pass the filter residue through a squeezer to obtain the remaining fresh juice, filter, combine all the filtrates and add gasoline, stir them thoroughly, put them in a separatory funnel, let stand for more than 3 hours, the liquid is divided into two layers.

[0045] (3) The upper layer is the oil layer, which is dark green, the lower layer is yellow liquid, called the water layer, the middle layer is suspended above the water layer, and the lower layer is called the gray sedimentary layer. Filter the water layer, gray precipitation layer, and oil layer separately, combine the filter residues, wash with distilled water and drain, and dry at 100°C to obtain 81.2 g of grass protein; recover ethanol and gasoline from the oil layer under redu...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for extracting chlorophyll from wild deyeuxia angustifolia forage grass and converting the chlorophyll into sodium copper chlorophyllin, and particularly to a new method, wherein the new method comprises adopting a two-step method to extract chlorophyll and grass protein, carrying out alkaline solution saponification on the paste chlorophyll, and replacing copper with sodium copper chlorophyllin. The method has the following advantages that: 1, the two-step method is adopted to extract chlorophyll, such that the time is shortened compared with the conventional method, and the active ingredient chlorophyll extraction rate is improved; 2, wild forage grass deyeuxia angustifolia in Daxinganling is adopted as the raw material, such that the local wild resources are comprehensively utilized, and two substances of grass protein and sodium copper chlorophyllin can be obtained; and 3, the method of the present invention has characteristics of simpleness, easy operation, simple process, low investment, and low production cost, and is suitable for large-scale production. With the method of the present invention, the two-step extraction method is adopted to extract chlorophyll, the grass is grinded and extracted, the filter residue is soaked and extracted to obtain the glass protein and the paste chlorophyll, alkaline solution saponification is performed, acidification is performed after extraction is performed, copper is replaced, and purifying is performed to obtain the sodium copper chlorophyllin.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting chlorophyll from wild camphor grass and converting it into sodium copper chlorophyllin, in particular to a two-step method for extracting chlorophyll and grass protein, and saponifying pasty chlorophyll with lye , a new method for copper substitution into sodium copper chlorophyllin. Background technique: [0002] Daxinganling small-leaf camphor grass, also known as thatch grass, is a fast-growing grass with thin stems and many leaves. It generally grows wild. It grows very lushly in forest areas, ditches and ponds, and low-lying places. In some places, it grows in patches. Like a planted wheat field, the yield is very high. According to measurements, camphor lobata contains 10% to 16% of crude protein, 1% to 2% of crude fat, and more than 30% of nitrogen-free extract, which is a good feed for dairy cows. Chlorophyll is insoluble in water but solu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07D487/22C07K14/415C07K1/14
Inventor 姚德坤姚德利张亚红万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap