Method for extracting phospholipid-rich krill oil from Antarctic krill

Abstract

Disclosed is a method for extracting phospholipid-rich krill oil from Antarctic krill. The method includes using the Antarctic krill or Antarctic krill head shells as raw materials, subjecting the raw materials to pretreatment such as heating for enzyme-killing and sterilization and alkaline solution soaking, performing water removing, drying and smashing, using organic solvents to extract krill powders, performing filtering, subjecting separated extracting solutions to rotary evaporation, and removing the residual organic solvents to obtain the Antarctic krill oil with phospholipid contents over 40%. The method for extracting the phospholipid-rich krill oil from the Antarctic krill is simple in operating, the obtained phospholipid-rich krill oil is cheap, safe, environment-friendly, highin the phospholipid contents, low in acid values and good in liquidity, and the method is capable of improving stability of the phospholipid oil, improving qualities of the phospholipid oil and greatly improving comprehensive utilization rate of the Antarctic krill and can be used as a simple and practicable method for extracting oil and particularly the phospholipid-rich oil from the Antarctic krill.

Description

technical field

[0001] The invention relates to a method for extracting krill oil from Antarctic krill, in particular to a method for extracting krill oil rich in high phospholipid content. Background technique

[0002] Antarctic krill is one of the biological species with the largest resources on the earth. According to statistics, the biomass of Antarctic krill resources is conservatively estimated to be about 650 to 1 billion tons. Antarctic krill has attracted increasing attention because of its huge biological reserves, high nutritional value and special status in the Antarctic ecosystem. Antarctic krill not only contains a large amount of high-quality protein, but also contains lipids accounting for about 2% to 5% of wet weight. The content of phospholipids in lipids is relatively high, and it contains more omega-3 series polyunsaturated fatty acids, mainly Fatty acid types include: DHA9.3% ~ 14.2%, EPA15.0% ~ 20.5%, linoleic acid 2.4% ~ 3.8%, linolenic acid 1.3% ~ 3....

Examples

Example Embodiment

[0018] Example 1

[0019] (1) Heating: Antarctic krill is boiled and heated at 80°C for 10 minutes;

[0020] (2) Soaking: soak the heated Antarctic krill in sodium acetate solution for 0.5 hours, the mass volume ratio of Antarctic krill to the solution is 1:10, the concentration used in the solution is 1%, and the soaking process temperature is 20°C, stir once every 10 minutes;

[0021] (3) Washing: wash the soaked Antarctic krill until neutral, and drain the water;

[0022] (4) Drying: Freeze-dry the washed Antarctic krill at -50°C;

[0023] (5) Crushing: after drying, the Antarctic krill is crushed to 80 mesh krill powder, and the crushing time is 1 minute;

[0024] (6) Extraction: Extract the pulverized krill meal with 95% ethanol solution organic solvent at 30°C, the mass volume ratio of Antarctic krill to solvent is 1:5, and the extraction time is 6 hours ;

[0025] (7) Filtration: filter and separate the leached solution;

[0026] （ 8) Solvent evaporation: Rotate ...

Example Embodiment

[0027] Example 2

[0028] (1) Heating: Antarctic krill is boiled and heated at 100°C for 5 minutes;

[0029] (2) Soaking: soak the heated Antarctic krill in sodium ascorbate solution for 1 hour, the mass volume ratio of Antarctic krill to the solution is 1:5, the concentration used in the solution is 1%, and the soaking process temperature is 15°C, stirring once every 30 minutes;

[0030] (3) Water washing: Wash the soaked Antarctic krill until neutral, remove the head of the washed Antarctic krill to obtain the shrimp head shell, and drain the water;

[0031] (4) Drying: Freeze-dry the washed Antarctic krill shrimp heads at -50°C;

[0032] (5) Crushing: After drying, the Antarctic krill shrimp head shells are crushed to 50 mesh krill head shell powder, and the crushing time is 5 minutes;

[0033] (6) Extraction: Extract the crushed krill head shell powder with 95% ethanol solution organic solvent at 30°C, the mass volume ratio of Antarctic krill to solvent is 1:10, and the...

Example Embodiment

[0036] Example 3

[0037] (1) Heating: Antarctic krill is boiled and heated at 95°C for 8 minutes;

[0038] (2) Soaking: soak the heated Antarctic krill in trisodium citrate solution for 2 hours, the mass volume ratio of Antarctic krill to the solution is 1:10, the concentration used in the solution is 1%, the soaking process The temperature is 20°C, stirring once every 30 minutes;

[0039] (3) Washing: wash the soaked Antarctic krill until neutral, and drain the water;

[0040] (4) Drying: The washed Antarctic krill is dried by electric blast at 40°C;

[0041] (5) Crushing: after drying, the Antarctic krill is crushed to 20 mesh krill powder, and the crushing time is 5 minutes;

[0042] (6) Extraction: The pulverized krill powder is extracted with an organic solvent of ethanol solution with a concentration of 98% at 10°C. The mass volume ratio of Antarctic krill to solvent is 1:10, and the extraction time is 6 hours. ;

[0043] (7) Filtration: filter and separate the l...

technical field

[0001] The invention relates to a method for extracting krill oil from Antarctic krill, in particular to a method for extracting krill oil rich in high phospholipid content. Background technique

[0002] Antarctic krill is one of the biological species with the largest resources on the earth. According to statistics, the biomass of Antarctic krill resources is conservatively estimated to be about 650 to 1 billion tons. Antarctic krill has attracted increasing attention because of its huge biological reserves, high nutritional value and special status in the Antarctic ecosystem. Antarctic krill not only contains a large amount of high-quality protein, but also contains lipids accounting for about 2% to 5% of wet weight. The content of phospholipids in lipids is relatively high, and it contains more omega-3 series polyunsaturated fatty acids, mainly Fatty acid types include: DHA9.3% ~ 14.2%, EPA15.0% ~ 20.5%, linoleic acid 2.4% ~ 3.8%, linolenic acid 1.3% ~ 3....

Examples

Example Embodiment

[0018] Example 1

[0019] (1) Heating: Antarctic krill is boiled and heated at 80°C for 10 minutes;

[0020] (2) Soaking: soak the heated Antarctic krill in sodium acetate solution for 0.5 hours, the mass volume ratio of Antarctic krill to the solution is 1:10, the concentration used in the solution is 1%, and the soaking process temperature is 20°C, stir once every 10 minutes;

[0021] (3) Washing: wash the soaked Antarctic krill until neutral, and drain the water;

[0022] (4) Drying: Freeze-dry the washed Antarctic krill at -50°C;

[0023] (5) Crushing: after drying, the Antarctic krill is crushed to 80 mesh krill powder, and the crushing time is 1 minute;

[0024] (6) Extraction: Extract the pulverized krill meal with 95% ethanol solution organic solvent at 30°C, the mass volume ratio of Antarctic krill to solvent is 1:5, and the extraction time is 6 hours ;

[0025] (7) Filtration: filter and separate the leached solution;

[0026] （ 8) Solvent evaporation: Rotate ...

Example Embodiment

[0027] Example 2

[0028] (1) Heating: Antarctic krill is boiled and heated at 100°C for 5 minutes;

[0029] (2) Soaking: soak the heated Antarctic krill in sodium ascorbate solution for 1 hour, the mass volume ratio of Antarctic krill to the solution is 1:5, the concentration used in the solution is 1%, and the soaking process temperature is 15°C, stirring once every 30 minutes;

[0030] (3) Water washing: Wash the soaked Antarctic krill until neutral, remove the head of the washed Antarctic krill to obtain the shrimp head shell, and drain the water;

[0031] (4) Drying: Freeze-dry the washed Antarctic krill shrimp heads at -50°C;

[0032] (5) Crushing: After drying, the Antarctic krill shrimp head shells are crushed to 50 mesh krill head shell powder, and the crushing time is 5 minutes;

[0033] (6) Extraction: Extract the crushed krill head shell powder with 95% ethanol solution organic solvent at 30°C, the mass volume ratio of Antarctic krill to solvent is 1:10, and the...

Example Embodiment

[0036] Example 3

[0037] (1) Heating: Antarctic krill is boiled and heated at 95°C for 8 minutes;

[0038] (2) Soaking: soak the heated Antarctic krill in trisodium citrate solution for 2 hours, the mass volume ratio of Antarctic krill to the solution is 1:10, the concentration used in the solution is 1%, the soaking process The temperature is 20°C, stirring once every 30 minutes;

[0039] (3) Washing: wash the soaked Antarctic krill until neutral, and drain the water;

[0040] (4) Drying: The washed Antarctic krill is dried by electric blast at 40°C;

[0041] (5) Crushing: after drying, the Antarctic krill is crushed to 20 mesh krill powder, and the crushing time is 5 minutes;

[0042] (6) Extraction: The pulverized krill powder is extracted with an organic solvent of ethanol solution with a concentration of 98% at 10°C. The mass volume ratio of Antarctic krill to solvent is 1:10, and the extraction time is 6 hours. ;

[0043] (7) Filtration: filter and separate the l...