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Method for transforming stevioside into rebaudioside E

A technology of stevioside and aspergillus niger is applied in the field of food chemical industry to achieve the effect of low cost and easy amplification

Inactive Publication Date: 2012-11-07
成都南诺格生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] So far, there is no report on the production of Rebaudioside E (Rebaudioside E) by biotransformation with sucrose as the sugar donor by microbial fermentation using stevioside (Stevioside), a single component of steviol glycosides, as a raw material

Method used

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  • Method for transforming stevioside into rebaudioside E
  • Method for transforming stevioside into rebaudioside E
  • Method for transforming stevioside into rebaudioside E

Examples

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Embodiment Construction

[0033] In a specific embodiment, stevioside (Stevioside) is abbreviated as ST, rebaudioside E (Rebaudioside E) is abbreviated as RE, and sucrose (Sucrose) is abbreviated as SU.

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Abstract

The invention discloses a method for transforming stevioside into rebaudioside E. The stevioside is transformed into the rebaudioside E by using aspergillns niger II which is added with a sugar source. According to the method, the stevioside is used as a raw material to obtain a transformation rate of the rebaudioside E, the transformation rate of the rebaudioside E is larger than and identical to 70%, the rebaudioside E content in solid matters can at least reach to 60%, after a further separation and purification, the rebaudioside E content can reach to 80% and is even larger than 90%, the rebaudioside E is free from extraneous odour, similar to cane sugar and capable of being used as a sweetener independently, the rebaudioside E production cost is low, and the method is easy to magnify and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of food chemical industry, and in particular relates to a method for converting stevioside into rebaudioside E. Background technique [0002] Steviol glycosides (Steviol glycosides) is a general term for stevia extracts. Its sweet ingredients include stevioside (Stevioside), rebaudioside A, B, C, D, E (Rebaudioside A / B / C / D / E), steviolbioside (Steviolbioside), dulcoside A (Dulcoside A), etc. In traditional steviol glycosides, stevioside accounts for about 66% of the total glycosides, rebaudioside A accounts for about 22% of the total glycosides, rebaudioside C accounts for about 9% of the total glycosides, and dulcoside A accounts for about 2% of the total glycosides, and the content of other components is very small. The structural formula of the main components of steviol glycosides is as follows: [0003] [0004] Stevioside Rebaudioside A Rebaudioside C [0005] [0006] Rebaudioside E Rebaudioside ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/56C12R1/685
Inventor 李沁桐欧阳劲
Owner 成都南诺格生物科技有限责任公司
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