Herba houttuyniae aboveground part extract and detection method thereof

A detection method and grassland technology, applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of not fully reflecting the quality of medicinal materials, measurement errors, etc.

Active Publication Date: 2014-07-02
ZHEJIANG GUOJING PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At the same time, when using liquid phase to detect the quality of Chinese medicinal materials, most of them use reference substances or fingerprints for detection. Among them, when using reference substances for detection, they cannot fully reflect the quality of medicinal materials; while using fingerprints, although they can fully reflect the quality of medicinal materials. Quality, however, may also be due to differences in various factors such as detection instruments, mobile phases, and environmental conditions, resulting in measurement errors

Method used

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  • Herba houttuyniae aboveground part extract and detection method thereof
  • Herba houttuyniae aboveground part extract and detection method thereof
  • Herba houttuyniae aboveground part extract and detection method thereof

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Effect test

Embodiment 1

[0091] Embodiment 1 Preparation of Houttuynia cordata aerial part extract of the present invention

[0092] Take the aerial part of fresh Houttuynia cordata, decoct twice with water, 0.5 hours each time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.18-1.20 (60-80°C), add ethanol until it contains The alcohol content is 70%, stir well, let it stand for 24 hours, filter, and recover the ethanol from the filtrate under reduced pressure and concentrate to an appropriate amount (including 5g / ml of raw medicinal materials). Take 5ml of the solution onto D101 macroporous adsorption resin (inner diameter 1cm, length 10cm), absorb, elute with water until colorless, then elute with 70% ethanol until colorless, collect the 70% ethanol eluate, evaporate to dryness on a water bath, The residue is dissolved with 70% methanol, filtered, and after recovering the solvent, the aerial part extract of Houttuynia cordata of the present inven...

Embodiment 2

[0093] Embodiment 2 The detection method of Houttuynia cordata aerial part of the present invention

[0094] (1) Preparation of reference solution

[0095] Take an appropriate amount of quercitrin reference substance, weigh it accurately, add methanol to make a solution containing 0.1633mg per 1ml, and use it as the reference substance solution.

[0096] Take appropriate amount of hyperin and chlorogenic acid reference substances respectively, weigh them accurately, add methanol to make a solution containing 0.0881 mg of hyperin and 0.1157 mg of chlorogenic acid per 1 ml, as the reference solution.

[0097] (2) Preparation of the test solution

[0098] Take the aerial part of fresh Houttuynia cordata, decoct twice with water, 0.5 hours each time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.18-1.20 (60-80°C), add ethanol until it contains The alcohol content is 70%, stir well, let it stand for 24 hours, filter, and rec...

Embodiment 3

[0114] The investigation of embodiment 3 detection method of the present invention

[0115] (1) Investigation of chromatographic conditions

[0116] (1) Screening test of mobile phase

[0117] ①Preparation of reference solution

[0118] Take an appropriate amount of quercitrin reference substance, weigh it accurately, add methanol to make a solution containing 0.1633mg per 1ml, and use it as the reference substance solution.

[0119] Take appropriate amount of hyperin and chlorogenic acid reference substances respectively, weigh them accurately, add methanol to make a solution containing 0.0881 mg of hyperin and 0.1157 mg of chlorogenic acid per 1 ml, as the reference solution.

[0120] ②Preparation of the test solution

[0121] Take the aerial part of fresh Houttuynia cordata, decoct twice with water, 0.5 hours each time, combine the decoction, filter, concentrate the filtrate to a clear paste with a relative density of 1.18-1.20 (60-80°C), add ethanol until it contains T...

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Abstract

The invention provides an aboveground part extract and a detection method thereof. The extract is detected via an HPLC (High Performance Liquid Chromatography) fingerprint method. The herba houttuyniae aboveground part extract covers multi-class active components, is unambiguous is material basis, can serve as a new standard control for medicinal material quality detection, and more facilitates quality control to the herba houttuyniae aboveground part. According to the invention, the optimal detection method of the herba houttuyniae aboveground part is achieved via a comprehensively surveying sample solution preparation method and color spectrum conditions. When the detection method is applied, a base line is stable, chromatographic peak shape and resolution are favorable, and meanwhile, flavonoid and organic acid chemical compounds of the herba houttuyniae can be synchronously detected, provides a reliable method for quality detection of the herba houttuyniae aboveground part, and ensures medicinal material quality of the herba houttuyniae aboveground.

Description

technical field [0001] The invention relates to a method for detecting the aerial part of Houttuynia cordata. Background technique [0002] Houttuynia cordata is the fresh whole herb or dry aerial part of Houttuynia cordata Thunb., a plant of the family Tribaceae. It is pungent in taste and slightly cold in nature, and it belongs to the lung meridian. For the treatment of pulmonary carbuncle and vomiting of pus, phlegm-heat dyspnea and cough, dysentery, pyretic stranguria, carbuncle sore tumefacting virus. [0003] Pharmacological studies have found that Houttuynia cordata has a strong antibacterial effect on Staphylococcus aureus, Shigella and other bacteria; Houttuynia cordata decoction has an inhibitory effect on viruses, and its non-volatile oil part can significantly prevent influenza virus; Grass decoction can also enhance the phagocytosis of white blood cells and has an immune-enhancing effect; the quercetin contained in Houttuynia cordata can expand renal blood vess...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 胡思玉余启波张德奎伏思颖何中燕王利春梁隆程志鹏
Owner ZHEJIANG GUOJING PHARM CO LTD
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