Time-of-flight-mass spectrometry (TOF-MS) fingerprint spectrum determination method for Relinqing granules and Polygonum capitatum
A technology of fingerprint and determination method, which is applied in the field of TOF-MS fingerprint determination of Relinqing Granules and Polygonum cephalosporin
Active Publication Date: 2014-05-14
GUIZHOU WARMEN PHARMA
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[0009] However, regarding the quality evaluation of Polygonum cephalosporins, for example, the quality evaluation of Polygonum cephalosporins in terms of the difference in origin, or the identification of the authenticity of the medicinal materials, as well as the extracts or preparations prepared from Polygonum cephalosporins, such as Relinqing granules, So far there is no applicable routine inspection method to control the quality of samples as a whole
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Embodiment 1
[0084] Example 1: UPLC-TOF-MS fingerprint analysis of Polygonum cephalum medicinal materials, extracts and preparations
[0085] In this example, UPLC-TOF-MS (UPLC of Waters Company; Q-TOF-MS of Bruker Company) fingerprint analysis was carried out on Polygonum cephalum medicinal materials, extracts and Relinqing preparations provided by the applicant, and the medicinal materials and extracts were identified. and the main ingredients in the preparation. The identification results showed that the medicinal materials of Polygonum cephalum mainly contained flavonoids and polyphenols. The chemical composition of the alcohol extraction extract of medicinal materials is very close to that of medicinal materials, while the composition of the water extraction extract has obvious chemical changes, mainly manifested in the hydrolysis and condensation of gallic tannin components (such as proanthocyanidins B1, B2 , B3, B4, etc.) loss.
[0086] 1. Samples, serial numbers and methods o...
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Abstract
The invention relates to a fingerprint spectrum determination method for Relinqing granules and Polygonum capitatum, in particular to a method for determining a fingerprint spectrum of a Polygonum capitatum drug, an extract of the Polygonum capitatum drug or a drug composition which comprises the extract, by ultra-high performance liquid chromatography-time-of-flight-mass spectrometry (UPLC-TOF-MS). The method comprises the following steps of: (i) test solution preparation: weighing an appropriate amount of sample, adding the sample into a methanol water solution to prepare a suspension, and performing ultrasonic treatment; (ii) providing UPLC-TOF-MS chromatographic analysis and mass spectrometry conditions; and (iii) injecting the sample in the step (i) into a liquid chromatograph, determining under the conditions in the step (ii), and thus obtaining a UPLC-TOF-MS total ion chromatogram of the sample. The method can be effectively applied to quality evaluation of the Polygonum capitatum drug, the extract of the Polygonum capitatum drug or a preparation.
Description
technical field [0001] The present invention relates to the field of traditional Chinese medicines, in particular to the field of quality evaluation of traditional Chinese medicines, in particular to a method for evaluating the quality of Relinqing granules and their raw materials, Polygonum cephalosporins, and in particular to the determination of the fingerprints of Relinqing granules and their raw materials, Polygonum cephalosporins The method, more particularly relates to a method for evaluating the quality of Relinqing granules and the medicinal material Polygonum cephalosporin by ultra-high performance liquid chromatography-time-of-flight-mass spectrometry (in the present invention, it may be referred to as UPLC-TOF-MS for short). method. Background technique [0002] Polygonum capitatum is the dried whole herb or aboveground part of Polygonum capitatum Buch.-Ham.ex D.Don, a plant of the Polygonaceae family. Antibacterial and anti-inflammatory, diuretic and stranguria...
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IPC IPC(8): G01N30/02G01N30/06G01N30/36
Inventor 张丽艳梁斌姜志宏李孟林谢宇唐靖雯潘梅
Owner GUIZHOU WARMEN PHARMA