Determination method of fingerprint spectrums of different-concentration ethanol extracts of polygonum capitatum
The technology of a fingerprint spectrum and a determination method is applied in the field of determination of the fingerprint spectrum of the ethanol extracts of Polygonum vulgaris with different concentrations, and can solve the problems of complex preparations and the like.
Active Publication Date: 2018-06-29
GUIZHOU WARMEN PHARMA
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The situation with compound preparations is more complicated
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Embodiment 1
[0039] Example 1: UPLC-TOF-MS fingerprint analysis of Polygonum cephalum medicinal materials, extracts and preparations
[0040] In this embodiment, UPLC-TOF-MS (UPLC of Waters company or 1290 Infinity ultra-high performance liquid phase of Agilent company of the United States) was carried out on the ethanol extract or water extract of different concentrations of Polygonum cephalosa provided by the applicant or its further preparation, that is, the hot shower preparation. Chromatography system; Bruker company Q-TOF-MS, or Agilent 6230TOF mass spectrometry system) fingerprint analysis to identify the main components in the extract and / or preparation. The identification results showed that the extract of Polygonum cephalosa mainly contained flavonoids and polyphenols.
[0041] 1. Materials
[0042] 30%, 50%, 70%, 95% ethanol extract, water extract 5 samples and 5 standard substances (ellagic acid, gallic acid, quercetin, protocatechuic acid, hyperin ) are provided by Guiya...
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Abstract
The invention relates to a determination method of fingerprint spectrums of different-concentration ethanol extracts of polygonum capitatum. The determination is performed according to an ultra-high performance liquid chromatography-flight time-gas chromatography-mass spectrometry method. The method comprises the following steps of (i) test solution preparation: weighting a proper number of polygonum capitatum extracts or medicine composition powder containing the polygonum capitatum extracts; adding a methanol water solution for preparing a turbid solution; performing ultrasonic treatment andfiltering; performing dilution in necessary; using obtained materials as test samples for detection; (ii) chromatogram and mass spectrum analysis conditions: using a TOF-MS mass spectrograph; using 0.1-percent formic acid water solution as a flowing phase A and 0.1-percent formic acid acetonitrile solution as a flowing phase B; in 25 to 40 minutes, performing elution according to a procedure of lowering the ratio of the flowing phase A from 95 percent to 0 percent and correspondingly raising the ratio of the flowing phase B from 5 percent to 100 percent; (iii) injecting the test specimen in the step (i) into a liquid chromatograph; performing determination according to the conditions of the step (ii); obtaining the UPLC-TOF-MS total ion flow chromatogram of a test specimen. The method provided by the invention has good effects.
Description
technical field [0001] The present invention relates to the field of traditional Chinese medicines, in particular to the field of quality evaluation of traditional Chinese medicines, in particular to a method for evaluating the quality of Relinqing granules and their raw materials, Polygonum cephalosporins, and in particular to the extraction of Relinqing granules and their raw materials, Polygonum cephalosporins, with different concentrations of ethanol The method for determining the fingerprint of a substance, more particularly relates to a method for analyzing Relinqing granules or medicinal material heads by ultra-high performance liquid chromatography-time-of-flight-mass spectrometry (referred to as UPLC-TOF-MS in the present invention). A method for determining the fingerprints of the ethanol extracts of different concentrations of Polygonum japonica, and a method for evaluating the quality of Polygonum cephalosporin. The quality control of ethanol extracts with differen...
Claims
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IPC IPC(8): G01N30/00G01N30/02
CPCG01N30/00G01N30/02
Inventor 梁斌杨槐张丽艳唐靖雯卢礼平姜志宏谢立敏王静蓉周雯
Owner GUIZHOU WARMEN PHARMA