Method for identifying green peel, orange peel, citrus aurantium and citrus aurantium based on chemical taxonomy and uplc-tof-ms

Abstract

The invention belongs to the technical field of medicine detection, and particularly relates to a method for identifying green tangerine peel, dried tangerine peel, fructus aurantii immaturus and fructus aurantii based on a chemical classifying method and UPLC-Tof-MS (ultra performance liquid chromatography- time of flight-mass spectrometry). Compared with the prior art, the method for identifying green tangerine peel, dried tangerine peel, fructus aurantii immaturus and fructus aurantii based on the chemical classifying method and the UPLC-Tof-MS has the advantage that by utilizing the high-flux sample analyzing capacity of metabonomics technique and the strong mathematical statistics method, the different varieties and the markers of chemical components for mutual distinguishing in / between growth phases are found to complete reasonable chemical classifying, so that the abstract efficiency description is objectively expressed by the qualitative and quantitative difference of metabolites, the possible action mechanism of traditional Chinese medicine playing different efficacy is reversed, the matter foundations of traditional Chinese medicines of different citrus types are disclosed, and the methodology reference is provided for establishing a new traditional Chinese medicine quality evaluation system.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a method for identifying green peel, orange peel, citrus aurantium and citrus aurantium based on a chemical classification method and UPLC-Tof-MS. Background technique [0002] Rutaceae (Rutaceae) Citrus (Citrus) is the world's largest fruit tree species, in the world's 100 fruits, its planting area and output both rank first, Citrus plants are domestic and foreign research in the fields of food science, nutrition and pharmacy hotspot. It is cultivated in more than 140 countries and regions in the world, mainly concentrated in Brazil, China, the United States and the countries along the Mediterranean Sea. There are 20 provinces (cities) in my country where Citrus plants are cultivated, and its planting area and output rank first in the world. . The fresh fruit of Citrus genus can be eaten directly and has high nutritional value, and its extract can be used as ...

AI Technical Summary

Problems solved by technology

Traditional classification methods include morphological classification based on phenotypic traits and gene classification based on DNA sequences. However, it is difficult to perform reasonable quality control on traditional Chinese medicines with only these two classification methods. For example, different growth stages of the same species Traditional Chinese medicines can hardly be identified by gene classification method, while the traditional Chinese medicines of orange genus Qingpi / Chenpi, Aurantium Fructus / Aurantium Fructus are all derived from the same source but in different growth stages.

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