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Method for biological purification of Mongolian gerbil

A long-clawed gerbil, bio-purification technology, applied in animal husbandry, etc., can solve the problem of biological purification of long-clawed gerbils

Inactive Publication Date: 2012-12-12
ZHEJIANG ACAD OF MEDICAL SCI
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Problems solved by technology

At present, there is no domestic research report on the biological purification of long-clawed gerbils, nor the selection of pregnant mice and the judgment of the labor period, aseptic caesarean section, milk substitutes for long-clawed gerbils, IVC feeding management and microbial detection. Written records of operating techniques in various aspects such as technology

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  • Method for biological purification of Mongolian gerbil
  • Method for biological purification of Mongolian gerbil
  • Method for biological purification of Mongolian gerbil

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Embodiment 1

[0037] Key technologies in the selection of gerbil pregnant mice and the judgment of the laboring period, sterile caesarean section, milk replacement of gerbil pups, IVC feeding management and microbial detection technology.

[0038] 1. Selection of pregnant mice and judgment of labor period

[0039] The gerbil is different from rats and mice. The gerbil is a "monogamous" long-term cohabitation mode of reproduction. Therefore, the 3-month-old gerbil of ordinary grade is selected for cohabitation and mating with a male-to-male ratio of 1:1. The gestation period of mice is 19-21 days, and the gestation period of gerbils is 24-26 days. Therefore, the mating of milk substitute ICR mice is generally 3-4 days later than the mating time of caesarean section mothers. Delivery within 12-36 hours before surgery. It is not easy to see obvious vaginal plugs after mating, and it is difficult to estimate the expected date of delivery of the long-clawed gerbil by finding the vaginal plug. ...

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Abstract

The invention discloses a method for biological purification of Mongolian gerbils. The method comprises the following steps of: selecting young Mongolian gerbils which are born through cesarean section under a germ-free condition, using ICR lactating mice as foster mother mice of the young Mongolian gerbils to provide breast milk or manually feeding milk to the young Mongolian gerbils, starting to add mouse feed powder containing rabbit milk to the young Mongolian gerbils which are 17-day-old to 20-day-old, keeping the content of the rabbit milk to be 60-70ml per 100g of mouse feed powder, and safely weaning the young Mongolian gerbils from milk after the body weight of each 25-day-old to 30-day-old young Mongolian gerbil is 16g to obtain weaned young Mongolian gerbils; and conducting detection of microorganisms such as mycoplasma and Tyzzer's organisms on mother Mongolian gerbils and the young Mongolian gerbils, putting acceptable weaned young Mongolian gerbils into a barrier system for separate feeding, tracing and detecting the microorganisms such as mycoplasma and Tyzzer's organisms on two generations of Mongolian gerbils, and using acceptable Mongolian gerbils as clean-grade Mongolian gerbils. By using the method, the microorganism grade of the Mongolian gerbils is improved to a clean grade from a conventional grade.

Description

technical field [0001] The invention relates to the field of biological purification of experimental animals, in particular to a method for biological purification of gerbils. Background technique [0002] Biological purification of experimental animals refers to improving the quality level of microorganisms and parasites of the species and strains on the premise of retaining the genetic traits of the experimental animals. According to the degree of microbiological quality control or according to the degree of microbial purification, it can be divided into five categories: [0003] (1) Conventional animals: also known as first-class animals, animals with the lowest requirements for microbiological control, which require no pathogens of zoonotic diseases and severe animal infectious diseases. Because of its low price, it is commonly used in teaching experiments. [0004] (2) Clean animals: also known as secondary animals, except for the pathogens that should be excluded by ...

Claims

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Application Information

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IPC IPC(8): A01K67/02
Inventor 戴方伟卢领群宋晓明周莎桑萨晓婴李长龙马伟锋柯贤福楼琦
Owner ZHEJIANG ACAD OF MEDICAL SCI
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