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34 results about "Germ free condition" patented technology

Organisms or environments not contaminated by or associated with any viable microorganisms.

Novel lithotomy position surgery leg bracket

The invention relates to a novel lithotomy position surgery leg bracket. The technical scheme of the novel lithotomy position surgery leg bracket is that, according to the position characteristic of lithotomy position surgery, a traditional scheme that popliteal space of a leg is supported is changed, popliteal space supporting is replaced by shank supporting, and all untoward effects caused by the traditional popliteal space supporting are solved. A boot-shaped design completely according with the three-dimensional physiological curve of a shank can reduce the press on the popliteal space anda gastrocnemius muscle, and avoid the damage on popliteal space plexus and fibular nerves. Cantilever type leg supporting is adopted for adjusting positions needed in surgery at will, the surgery position is controlled by an adjusting handle, and the leg bracket can stay in a desired position at will. When the position is adjusted, no medical care personnel is needed for lifting and moving the leg of a patient, and even during the surgery, the medical care personnel can still easily adjust multiple angles in a germ free condition of the leg. The work intensity of the medical care personnel isreduced, the efficiency and safety of the surgery are improved, and the surgical results are improved.
Owner:奥克兰高分子医用材料(天津)有限公司

Domestication method for high temperature-resistant ethanol-tolerant aroma-producing yeast

The invention relates to the field of bioengineering technology and provides a domestication method for a high temperature-resistant ethanol-tolerant aroma-producing yeast. The method comprises the following steps: (1) screening a yeast strain with an excellent aroma-producing property from commercially available aroma-producing yeasts; (2) uniformly mixing an aroma-producing yeast bacterial liquid in a logarithmic phase with an aqueous sodium alginate solution at a room temperature under a germ-free condition, adding a mixed liquor into an aqueous calcium chloride solution to prepare a gel ball particle and immobilizing the gel ball particle at a temperature of 2 to 4 DEG C for 4 to 5 h so as to obtain an immobilized gel particle; and (3) subjecting an obtained immobilized aroma-producing yeast to high temperature-resistant ethanol-tolerant cyclic gradient domestication in an ethanol domestication medium and carrying out cyclic domestication a plurality of times so as to obtain the high temperature-resistant ethanol-tolerant aroma-producing yeast. According to the invention, a cyclic gradient domestication process is employed, so the domestication method is easy and convenient to operate and capable of screening a high temperature-resistant ethanol-tolerant aroma-producing yeast strain, the defect of poor high temperature and ethanol resistance of an aroma-producing yeast used in production of sesame-flavor liquor is overcome, and fermentation capacity is improved.
Owner:浙江省致中和生物健康食品研究院

Push-pull type sterile cell brush provided with replaceable brush head for endoscope

The invention discloses a push-pull type sterile cell brush provided with a replaceable brush head for an endoscope. The push-pull type sterile cell brush comprises a drainage tube, the brush head, a push-puller and a traction fiber, wherein the brush head is connected to the head of the drainage tube in a detachable way; a cell brush is arranged in the brush head; an opening is formed in the end, far away from the drainage tube, of the brush head; the traction fiber penetrates into the drainage tube, one end of the traction fiber is connected with the cell brush in a detachable way, and the other end of the traction fiber is connected with the push-puller; the cell brush is externally provided with a protective film; the head of the protective film is connected with the brush head, and the tail of the protective film is connected with the tail of the cell brush. The internal cell brush can be used for brushing bacteria living specimens and / or cell samples of an intestinal mucosal surface layer in a living intestinal cavity wall under the condition of being in conjunction with the drainage tube and the push-puller; furthermore, with the protection of the protective film, bacteria / cell brush pollution caused by the pollution to a channel of endoscopic biopsy forceps can be avoided. After the push-pull type sterile cell brush is used, the sterile cell brush head can be replaced, so that the cell brush can be repeatedly used in a germ-free condition; therefore, the collection credibility of samples of all intestinal tract parts can be maintained to the utmost extent while the cost is lowered.
Owner:JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV +1

Method for biological purification of Mongolian gerbil

InactiveCN102812920AAnimal husbandryMicroorganismClawed Jird
The invention discloses a method for biological purification of Mongolian gerbils. The method comprises the following steps of: selecting young Mongolian gerbils which are born through cesarean section under a germ-free condition, using ICR lactating mice as foster mother mice of the young Mongolian gerbils to provide breast milk or manually feeding milk to the young Mongolian gerbils, starting to add mouse feed powder containing rabbit milk to the young Mongolian gerbils which are 17-day-old to 20-day-old, keeping the content of the rabbit milk to be 60-70ml per 100g of mouse feed powder, and safely weaning the young Mongolian gerbils from milk after the body weight of each 25-day-old to 30-day-old young Mongolian gerbil is 16g to obtain weaned young Mongolian gerbils; and conducting detection of microorganisms such as mycoplasma and Tyzzer's organisms on mother Mongolian gerbils and the young Mongolian gerbils, putting acceptable weaned young Mongolian gerbils into a barrier system for separate feeding, tracing and detecting the microorganisms such as mycoplasma and Tyzzer's organisms on two generations of Mongolian gerbils, and using acceptable Mongolian gerbils as clean-grade Mongolian gerbils. By using the method, the microorganism grade of the Mongolian gerbils is improved to a clean grade from a conventional grade.
Owner:ZHEJIANG ACAD OF MEDICAL SCI

Grating filter membrane with sterilization indicating function

InactiveCN102151488ADirect identification of sterilization statusWith sterilization color change indicator functionSemi-permeable membranesLavatory sanitoryWater basedGrating
The invention discloses a grating filter membrane with a sterilization color-change indicating function, aiming at solving the problem that whether the filter membrane is sterilized can not be recognized and further the germ-free condition of the filter membrane can not be confirmed when microorganisms in liquid and air are separated by adopting a microporous membrane and being capable of conveniently counting colonies on the filter membrane. A grating line (2) on the filter membrane (1) in the invention is a water-based grating line. In order to realize the purpose of the invention, sterilizing color ink is printed on the surface of the filter membrane by adopting methods of silk-screen and the like, the ink and the filter membrane can have the same color and can have different colors, the grating line is changed from the original color into a sterilized color in color after being subjected to a sterilization mode suitable for indication ink and forms a remarkable color difference with the color of the filter membrane, therefore, the germ-free condition of the filter membrane can be confirmed and the microorganisms are conveniently counted. The invention can ensure the result of detecting the microorganisms, and can be widely applied to the detection of the microorganisms in liquid and air.
Owner:ZHEJIANG TAILIN BIOENG

Preservative-free biological activity application technology

The invention relates to the technical field of biological activity application, and discloses a preservative-free biological activity application technology, which comprises a mixed base material, distilled water, EM bacteria, an Rw bacterial agent, plant essential oil and an extract, protamine, tea polyphenol and propolis. The preservative-free biological activity application technology comprises the following specific operation steps: taking corresponding parts of the raw materials for later use; mixing the mixed base material and distilled water, and adding into a mixing tank to obtain a mixed solution; sequentially putting the plant essential oil, the extract, the protamine, the tea polyphenol and the propolis into the mixed solution; and adding EM bacteria and RW bacterial agent into the mixing tank for full reaction to obtain a hgih-activity biological reaction product. According to the preservative-free biological activity application technology, four groups of samples are respectively placed in a storage tank in a sterile state to be sealed and stored, a sampling experiment is carried out, the putrefaction degree of the biological active substances in the four samples is detected, therefore, the optimal proportion is obtained, and a relatively high preservative effect can also be achieved under the condition that the biological activity is maintained.
Owner:广州泊亚生物科技有限公司

Microbiological detection equipment based on biopharmacy

The invention discloses biological pharmacy-based microbiological detection equipment, the biological pharmacy-based microbiological detection equipment comprises a body and an extraction pipe arranged outside the body, and a disinfection sampling mechanism is arranged outside the body; the extraction pipe is slowly put down through a transmission wheel, so that alcohol sprayed out of a spray head can fully sterilize and disinfect the outer surface of the extraction pipe, meanwhile, the surface of the extraction pipe can be wiped through an alcohol cotton block, so that the cleaned and disinfected surface of the extraction pipe is cleaner, and through the design of a scraping plate, the scraping plate is convenient to clean. Alcohol residues left on the surface of the cleaned extraction pipe can be completely scraped, so that the surface of the extraction pipe entering the biopharmaceutical microorganism culture tank can be ensured to be in a sterile state; the situation that the extraction pipe is directly and manually inserted into the culture tank for sample extraction, so that strain microorganisms attached to the surface of the extraction pipe can be brought into the culture tank, and microbial liquid medicine for biological pharmacy in the culture tank is polluted is avoided.
Owner:郭庆芳

New energy garbage can with disinfection function

PendingCN113184410AReduce breedingTo achieve the purpose of dry and wet separationRefuse receptaclesRefuse cleaningDisinfectantEnvironmental hygiene
The invention provides a new energy garbage can with a disinfection function, and relates to the technical field of environment-friendly garbage cans, and the problems that the interior of a conventional garbage can is of a closed barrel-shaped structure, bred bacteria overflow and possibly damage the body health of people when the condition is serious, a high-cleanliness disinfection garbage can needs electronic induction, manufacturing cost of the garbage can is relatively high, and the garbage can is a high-frequency vulnerable object, so that energy waste is very liable to be caused can be solved. The new energy garbage can comprises a garbage can body. A supporting frame is vertically, upwards, longitudinally and fixedly installed in the middle of the upper end of the garbage can body. A herringbone shielding plate is fixedly installed at the upper end of the supporting frame. A disinfectant box is vertically installed in the position, in the middle of the supporting frame, of the garbage can body, and the lower end of the disinfectant box is arranged in an inner cavity of the garbage can body. The inner end of a liquid discharge pipe is located at the concave position of a flow guide bottom plate, so that the liquid discharge pipe performs liquid discharging finally in an independent state, the dry and sterile state of garbage is guaranteed, and the environmental sanitation is improved.
Owner:张玮

A push-pull sterile cell brush for endoscope with replaceable brush head

The invention discloses a push-pull type sterile cell brush provided with a replaceable brush head for an endoscope. The push-pull type sterile cell brush comprises a drainage tube, the brush head, a push-puller and a traction fiber, wherein the brush head is connected to the head of the drainage tube in a detachable way; a cell brush is arranged in the brush head; an opening is formed in the end, far away from the drainage tube, of the brush head; the traction fiber penetrates into the drainage tube, one end of the traction fiber is connected with the cell brush in a detachable way, and the other end of the traction fiber is connected with the push-puller; the cell brush is externally provided with a protective film; the head of the protective film is connected with the brush head, and the tail of the protective film is connected with the tail of the cell brush. The internal cell brush can be used for brushing bacteria living specimens and / or cell samples of an intestinal mucosal surface layer in a living intestinal cavity wall under the condition of being in conjunction with the drainage tube and the push-puller; furthermore, with the protection of the protective film, bacteria / cell brush pollution caused by the pollution to a channel of endoscopic biopsy forceps can be avoided. After the push-pull type sterile cell brush is used, the sterile cell brush head can be replaced, so that the cell brush can be repeatedly used in a germ-free condition; therefore, the collection credibility of samples of all intestinal tract parts can be maintained to the utmost extent while the cost is lowered.
Owner:JIANGSU PROVINCE HOSPITAL THE FIRST AFFILIATED HOSPITAL WITH NANJING MEDICAL UNIV +1

Preparation method of L-lysine sulfate and by-products thereof

The invention discloses a preparation method of L-lysine sulfate and by-products thereof, and belongs to the technical field of lysine production processes. The preparation method comprises the following steps: adjusting the pH value of a fermentation medium to 7.0-7.1 by using 20% NaOH, then continuously heating the medium by using steam to cause microbial death, inoculating lysine producing bacteria in a sterile state, carrying out fermentation at a temperature of 35-40 DEG C, a pH value of 6.8-7.0 and a DO value of 20-30% for 48-60 hours, after the fermentation is finished, putting the mixture of lysine and the substrate into an isoelectric extraction workshop, adjusting the pH value of the fermentation liquid to 5-6 by using sulfuric acid, filtering the treated fermentation liquid by using ceramic membrane equipment, performing triple-effect concentration on the filtered clear liquid, and performing granulation and drying by using a guniting granulation bed to obtain a finished product. According to the preparation method, the environmental protection problem is solved, the production processes are reduced, the process route is simple, the consumption of raw materials and auxiliary materials is low, the cost is low, and the produced lysine content is high.
Owner:黑龙江成福食品集团有限公司

Method for keeping rotary sealing cover head of PET product sterile

The invention relates to the technical field of sealing cover cleaning, and discloses a method for keeping a rotary sealing cover head of a PET product sterile. For solving the problem that the sanitation dead angle of the inner wall of a sealing cover head cannot be thoroughly eliminated in the prior art, a nozzle for washing and disinfecting the sealing cover head is additionally arranged around the sealing cover head on a production line, disinfectant is sprayed out of the nozzle, microorganisms hidden in the sealing cover head are killed, and the inner wall of the sealing cover head is kept sterile in the whole process. The method has the beneficial effects that on the basis of cleaning and disinfection of a conventional filling machine, the nozzle for washing and disinfecting the sealing cover head is additionally arranged, the microorganisms hidden in the sealing cover head can be effectively killed in the production process, and the inner wall of the sealing cover head can be kept in a sterile state in the whole process; according to the method, the needed transformation spare part price and the operation cost are both low, and the part transformation cost does not exceed 400 yuan; and the method can ensure that the disinfectant residue in the product meets a standard requirement.
Owner:HANGZHOU WAHAHA TECH +1

A separate intelligent refrigeration and fresh-keeping machine and its refrigeration and fresh-keeping method

Disclosed are a separation type intelligent refrigeration freshness retaining machine and a refrigeration freshness retaining method thereof. The separation type intelligent refrigeration freshness retaining machine comprises a cylindrical container used for containing protoplasmic beer, wherein the cylindrical container comprises an inner shell and an outer shell wound on the outer portion of the inner shell in a sleeving mode, a gap is kept between the inner wall of the outer shell and the inner shell, a first through hole and a second through hole are formed in the wall of the outer shell, the protoplasmic beer is stored in the inner shell, an interlayer for refrigeration is wound on the outer wall of the inner shell, the interlayer for refrigeration is communicated with one end of a first metal pipe and one end of a second metal pipe, and one end of the first metal pipe and one end of the second metal pipe are in the gap. According to the device, the defects that in the prior art, when a container which is formed integrally and contains the protoplasmic beer is inconveniently cleaned and maintained, in addition, the cleaning effect is poor, and the germ free condition cannot be ensured. In addition, the machine is combined with the refrigeration freshness retaining method of the machine, the temperature and pressure can be stably and efficiently controlled.
Owner:朱仁息

Pipettor suction nozzle box

PendingCN112169856AReliable sterilityPrevent automatic pop-upBurette/pipette supportsMicroorganismThermodynamics
The invention relates to the field of microorganism experiment instruments, in particular to a pipettor suction nozzle box. The box comprises a box body and a box cover, a bearing part is arranged inthe box body, a plurality of insertion holes are formed in the bearing part, insertion hole covers are arranged at the opening positions of the insertion holes, pipe bodies for containing suction nozzles are arranged in the insertion holes, film holes are formed in the insertion hole covers, and the positions of the film holes are covered with air purification films. According to the product, theinsertion hole covers are arranged at the openings of the pipe bodies by adopting a double-layer locking system, so that the insertion hole covers are effectively prevented from being automatically bounced off during high-pressure steam sterilization, and the cover sealing effect is very reliable. each suction nozzle is provided with an insertion hole cover, and each insertion hole cover is provided with a breathable film, so that the effect of high-pressure steam sterilization is guaranteed, air permeability and moisture permeability are achieved, it can be guaranteed that the suction nozzlesto be used are in a reliable sterile state during operation, and test errors caused by pollution and wetting due to the situation are avoided; and the insertion hole covers can be used repeatedly, sothat the manufacturing cost is reduced.
Owner:GUANGXI AGRI VOCATIONAL COLLEGE
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