The invention discloses a preparation method of L-lysine sulfate and by-products thereof, and belongs to the technical field of lysine production processes. The preparation method comprises the following steps: adjusting the pH value of a fermentation medium to 7.0-7.1 by using 20% NaOH, then continuously heating the medium by using steam to cause microbial death, inoculating lysine producing bacteria in a sterile state, carrying out fermentation at a temperature of 35-40 DEG C, a pH value of 6.8-7.0 and a DO value of 20-30% for 48-60 hours, after the fermentation is finished, putting the mixture of lysine and the substrate into an isoelectric extraction workshop, adjusting the pH value of the fermentation liquid to 5-6 by using sulfuric acid, filtering the treated fermentation liquid by using ceramic membrane equipment, performing triple-effect concentration on the filtered clear liquid, and performing granulation and drying by using a guniting granulation bed to obtain a finished product. According to the preparation method, the environmental protection problem is solved, the production processes are reduced, the process route is simple, the consumption of raw materials and auxiliary materials is low, the cost is low, and the produced lysine content is high.