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A bio-fermentation method that can increase the protein content of cottonseed meal and can detoxify

A technology of biological fermentation and protein content, applied in fermentation, animal feed, animal feed, etc., can solve the problems of restricting the application of cottonseed meal, and achieve the effect of reducing the content of free gossypol, increasing the nutritional value and improving the taste.

Active Publication Date: 2017-10-17
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existence of these anti-nutritional factors, the application of cotton meal in livestock and poultry feed and aquatic feed is greatly restricted.

Method used

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  • A bio-fermentation method that can increase the protein content of cottonseed meal and can detoxify
  • A bio-fermentation method that can increase the protein content of cottonseed meal and can detoxify
  • A bio-fermentation method that can increase the protein content of cottonseed meal and can detoxify

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Improve the crude protein content of cottonseed meal and the screening of strains that degrade gossypol

[0019] Raw cottonseed meal is derived from the by-products of cottonseed after oil extraction; the weight ratio of raw material to water is 1:0.8, and the prepared Aspergillus niger CICC41057 (purchased from China Institute of Food and Fermentation Industry), Candida utilis CICC 31188 (purchased from China Food Fermentation Industry Research Institute), Saccharomyces cerevisiae (CGMCC No.2388), Bacillus licheniformis (CGMCC No.5094), Lactobacillus salivarius CICC 21807 (purchased from China Food Fermentation Industry Research Institute) hospital); the fermentation temperature is 30°C, and the fermentation time is 48 hours. The test results are shown in Table 1.

[0020] Table 1 Effects of different bacterial strains on the crude protein content and gossypol of cottonseed meal

[0021]

[0022] According to the results, Aspergillus niger and Candida ut...

Embodiment 2

[0023] Example 2 Screening of bacterial strains that improve the mouthfeel of cotton meal

[0024] Raw cotton meal is derived from the by-products of cottonseed after oil extraction; the weight ratio of raw material to feed water is 1:0.8, and the prepared Bacillus subtilis (CGMCC No.4628), acidophilus milk bacillus (CGMCC No.5093), Enterococcus faecalis (CGMCC No.5092), Bacillus pumilus (CGMCC No.4756), Bacillus licheniformis (CGMCC No.5094), Lactobacillus salivarius CICC 21807; fermentation temperature is 30 ℃, fermentation The time is 48 hours. The test results are shown in Table 2.

[0025] Table 2 Effects of different strains on the pH of cottonseed meal

[0026]

[0027] It can be seen from Table 2 that the pH of cotton meal fermented by Bacillus subtilis and Lactobacillus acidophilus is low, that is, more organic acids and other flavor substances are produced, so it is selected as the strain to improve the taste of cotton meal. Embodiment 3 Cotton Meal Biological ...

Embodiment 3

[0027] It can be seen from Table 2 that the pH of cotton meal fermented by Bacillus subtilis and Lactobacillus acidophilus is low, that is, more organic acids and other flavor substances are produced, so it is selected as the strain to improve the taste of cotton meal. Embodiment 3 Cotton Meal Biological Fermentation

[0028] Preparation of Aspergillus niger fermentation liquid: Inoculate Aspergillus niger into PDA slant medium, culture at 30°C for 72h, pick the mycelium on the slope, insert it into PDA liquid medium, and culture at 30°C for 16h with shaking at 150r / min.

[0029] Preparation of Candida utilis fermentation broth: insert Candida utilis into YPD slant medium, culture at 30°C for 48 hours, pick the lawn on the slant, insert it into YPD liquid medium, shake at 30°C, 150r / min Cultivate for 48h.

[0030] Preparation of Bacillus subtilis fermentation broth: Introduce Bacillus subtilis into LB slant medium, culture at 37°C for 24 hours, pick the bacterial lawn on the ...

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Abstract

The invention discloses a bio-fermentation method capable of increasing the protein content of cottonseed meal and capable of detoxification, comprising: 1) respectively preparing fermentation liquids of Candida utilis, Aspergillus niger, Bacillus subtilis and Lactobacillus acidophilus as strains; 2) Prepare raw cotton meal; 3) Add water to the raw material, the weight ratio of raw material to water is 1:0.5-1.2; 4) Inoculate the strains prepared in step 1), respectively Candida utilis 0.5%-10 %, Aspergillus niger 0.5%-10%, Bacillus subtilis 0.5%-10%, Lactobacillus acidophilus 0.5%-10%; The product directly obtained by the method of the invention can effectively degrade free gossypol in the cottonseed meal, improve the protein quality of the cottonseed meal, improve the taste of the cottonseed meal, thereby effectively improving the utilization rate of the cottonseed meal in various livestock and poultry feeds.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation and relates to a biological fermentation method capable of increasing the protein content of cottonseed meal and capable of detoxification. Background technique [0002] Feed protein can be divided into animal protein and plant protein according to the source of raw materials. Among them, animal protein such as fish meal, meat and bone meal and blood meal has similar amino acid composition to the amino acid required for the growth of livestock and poultry, so this protein is not only The absorption rate is high, and it also has the advantages of fast absorption and less pollution. However, my country is a country that is extremely short of animal protein resources such as fishmeal. In particular, high-quality fishmeal mainly relies on imports, and the price is much higher than that of vegetable protein. In addition, due to the safety of animal protein, in recent years, people More ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A23K10/12
Inventor 孙晓雯张军汪攀闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD