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DHA (docosahexaenoic acid)-rich microalgae powder and preparation method thereof

A technology of microalgae powder and preparation steps, applied in application, animal feed, animal feed, etc., can solve the problems of complex process, oxidative deterioration, etc., and achieve the effect of simple process, low water content and high DHA content

Active Publication Date: 2015-05-27
INNER MONGOLIA KINGDOMWAY PHARMA LTD +1
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Problems solved by technology

However, the process is complex. With DHA fermented liquid as raw material, it takes eight processes to obtain the product. The powder making process adopts traditional embedding technology, and the material is in a high temperature (180-190°C) state during the powder spraying process, which is also prone to occurrence Oxidative deterioration

Method used

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  • DHA (docosahexaenoic acid)-rich microalgae powder and preparation method thereof

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Embodiment Construction

[0026] The present invention will be further described below by taking the fermentation of dinoflagellates as an example, but it is not limited to the following examples and the range of process parameters in the examples.

[0027] (1) Fermentation and cultivation of DHA

[0028] Get the single colony of DHA-producing bacteria diflagellate diflagellate (Crypthecodiniumcohnii ATCC30556), inoculate into a 250ml shake flask with 50m1 seed culture medium, place the shake flask in a constant temperature shaker to activate the seeds, the temperature is 28°C, the rotation speed is 200rpm, Cultivate for 2 days to obtain a seed solution with high vigor; inoculate 10% of the inoculum into a 2L shake flask containing 400mL seed medium for expanded cultivation, and place the shake flask in a constant temperature shaker for cultivation at a temperature of 28°C and a rotation speed of 200rpm , cultivated for 2 days to obtain the secondary seed liquid; inoculated the secondary seed liquid in...

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Abstract

The invention discloses DHA (docosahexaenoic acid)-rich microalgae powder and a preparation method thereof. The DHA-rich microalgae powder is directly obtained by drying a DHA-rich microbial fermentation solution and is of faint yellow-brownish yellow granular powder, the particle size is 20-200 meshes, and the DHA content is not less than 20%. The preparation method comprises the following steps of: firstly performing microbial fermentation to produce DHA, adding an antioxidant into the obtained fermentation solution, performing flocculation, then performing plate and frame filtration to get a filter cake, performing vacuum freeze drying to get a dried solid with the water content of not more than 5%, pulverizing and granulating to get the microalgae powder. According to the preparation method disclosed by the invention, a vacuum freeze drying is utilized for preparing the DHA-rich fermentation solution into the DHA-rich microalgae powder, and the DHA in microalgae can be retained in a safe and high-efficient manner. The microalgae powder can be used as a feed additive, the DHA can be effectively enriched in animal bodies, and DHA-rich milk, eggs and meat can be further produced to meet the DHA intake needs of all types of people.

Description

technical field [0001] The invention relates to a microalgae powder and a preparation method thereof, in particular to a DHA-rich microalgae powder and a preparation method thereof. Background technique [0002] DHA (docosahexaenoic acid) is an extremely important omega-3 series highly unsaturated fatty acid, which is an important component of human cell membrane and nerve tissue. It plays an important role in ensuring the normal growth and development of the fetus, promoting the growth and development of infants, preventing cardiovascular diseases, inhibiting and treating certain cancers, and ensuring the normal work of the nervous system. [0003] "Chinese DHA Consumption Survey" shows that Chinese people's daily dietary intake of DHA is obviously insufficient. Many countries have established the daily recommended intake of DHA for the human body, the lowest intake is 160mg / day, and the highest is 400mg / day. The daily intake of DHA directly from food by the Chinese publi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/16
Inventor 刘华英徐鲁明王炳荣王跃飞王海彬韩雯吴轶詹光煌
Owner INNER MONGOLIA KINGDOMWAY PHARMA LTD
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