Method for depilating cilium on body surface of seawater infusorians
A technology of ciliates and seawater, applied in the field of zoological classification, can solve the problems of morphological taxonomy research and mouthparts observation, and achieve the effect of clean and thorough hair removal, low cost and high hair removal rate
Active Publication Date: 2014-09-17
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology
[0005] The technical problem to be solved in the present invention is to provide a method for removing the cilia on the body surface of seawater ciliates, so as to solve the technical problems that the mouthparts are difficult to observe and the research on morphological taxonomy is very difficult.
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[0020] In November 2010, a sea cucumber in a sea cucumber farm in Shandong Jiaonan developed ciliate disease. The sick sea cucumber was placed in an iced foam box and transported back to the Yellow Sea Fisheries Research Institute (Qingdao), Chinese Academy of Fishery Sciences. Body hair removal treatment.
[0021] Hair removal method: a. Place the obtained seawater ciliates in a 1500μl distilled water solution (ie a sodium chloride solution with a mass ratio of 30‰) dissolved in 0.045g of sodium chloride (NaCl), and then add 1500μl of a 16% ethanol solution , Make the concentration of ethanol in the solution 8%, stir evenly with a glass rod and let stand for 1~2 minutes;
[0022] b. Add 3000μl of 15‰ sodium chloride solution to make the concentration of ethanol in the solution 4%, stir it with a glass rod and let it stand for 1~2 minutes;
[0023] c. Add 6000μl of 15‰ sodium chloride solution to make the concentration of ethanol in the solution 2%, and stir evenly with a glass rod ...
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The invention provides a method for depilating cilium on the body surface of seawater infusorians, belonging to the field of zootaxy. The method comprises the following steps of: a, arranging obtained seawater infusorians in a sodium chloride solution with a weight ratio of 3%, and then adding an ethanol solution with a volume ratio of 10% and the same volume of the sodium chloride solution, so that the concentration of ethanol in the solution is 8% (volume ratio); uniformly stirring the mixed solution by using a glass rod, and standing the stirred solution for 1-2 min; b, adding a sodium chloride solution with the same volume and the weight ratio of 1.5%, so that the concentration of the ethanol in the solution is 4%; and uniformly stirring the solution by using the glass rod and standing for 1-2 min; and c, adding the same volume of the sodium chloride solution with the weight ratio of 1.5%, so that the concentration of the ethanol in the solution is 2%; and using the glass rod to uniformly stir, so that the depilation process can be completed. With the adoption of the method provided by the invention, the technical problem that the instrumenta cibaria of the infusorians are difficult to observe, and the morphological taxonomy research is very difficult can be solved. The method has great practical values for the accurate classification, the life history research and the prevention drug research of the seawater infusorians.
Description
Technical field [0001] The invention belongs to the field of zoology classification, and specifically relates to a method for depilating cilia on the body surface of seawater ciliates. Background technique [0002] Seawater ciliate disease is an important disease in marine aquaculture, which can cause diseases of many economic marine animals such as fish, prawns and sea cucumbers. The large number of parasites of some seawater shield ciliates can cause the death of cultured animals. The research on the etiology and control technology of seawater ciliates has become a hot research topic. [0003] Accurate classification of ciliates is the basis for conducting life history research and disease prevention and control. Parasitic ciliates are often due to special adaptations, usually high on both sides, dense cilia on the body surface without differentiation, mouthparts are usually located on one side of the body, and its structure is difficult to observe and distinguish after silver s...
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IPC IPC(8): G01N1/28
Inventor 荣小军李彬王岚廖梅杰张正王印庚陈贵平
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI