Method for depilating cilium on body surface of seawater infusorians
A ciliate and seawater technology, applied in the field of zoological classification, can solve problems such as difficulty in morphological taxonomy research, difficulty in observing mouthparts, etc., and achieve the effects of clean and thorough hair removal, low cost and high hair removal rate.
Active Publication Date: 2013-02-13
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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[0005] The technical problem to be solved in the present invention is to provide a method for removing the cilia on the body surface of seawater ciliates, so as to solve the technical problems that the mouthparts are difficult to observe and the research on morphological taxonomy is very difficult.
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[0020] In November 2010, ciliates occurred in japonicus in a japonicus farm in Jiaonan, Shandong Province. The diseased japonicus was placed in a foam box with ice and transported back to the Yellow Sea Fisheries Research Institute (Qingdao), Chinese Academy of Fishery Sciences for worming. Body hair removal treatment.
[0021] Hair removal method: a. Place the obtained seawater ciliates in 1500 μl of distilled aqueous solution dissolved with 0.045 g of sodium chloride (NaCl) (that is, a sodium chloride solution with a mass ratio of 30‰), and then add 1500 μl of 16% ethanol solution , so that the concentration of ethanol in the solution is 8%, stir evenly with a glass rod and let stand for 1 to 2 minutes;
[0022] b. Add 3000 μl of 15‰ sodium chloride solution, so that the concentration of ethanol in the solution is 4%, stir evenly with a glass rod and let it stand for 1 to 2 minutes;
[0023] c. Add 6000 μl of 15‰ sodium chloride solution to make the concentration of ethanol...
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The invention provides a method for depilating cilium on the body surface of seawater infusorians, belonging to the field of zootaxy. The method comprises the following steps of: a, arranging obtained seawater infusorians in a sodium chloride solution with a weight ratio of 3%, and then adding an ethanol solution with a volume ratio of 10% and the same volume of the sodium chloride solution, so that the concentration of ethanol in the solution is 8% (volume ratio); uniformly stirring the mixed solution by using a glass rod, and standing the stirred solution for 1-2 min; b, adding a sodium chloride solution with the same volume and the weight ratio of 1.5%, so that the concentration of the ethanol in the solution is 4%; and uniformly stirring the solution by using the glass rod and standing for 1-2 min; and c, adding the same volume of the sodium chloride solution with the weight ratio of 1.5%, so that the concentration of the ethanol in the solution is 2%; and using the glass rod to uniformly stir, so that the depilation process can be completed. With the adoption of the method provided by the invention, the technical problem that the instrumenta cibaria of the infusorians are difficult to observe, and the morphological taxonomy research is very difficult can be solved. The method has great practical values for the accurate classification, the life history research and the prevention drug research of the seawater infusorians.
Description
technical field [0001] The invention belongs to the field of zoological classification, and in particular relates to a depilation method of seawater ciliate body surface cilia. Background technique [0002] Sea ciliate is an important disease in mariculture, which can cause the disease of various economic marine animals such as fish, prawns and sea cucumbers, and a large number of parasitism of some sea shield ciliates can lead to the death of farmed animals. The etiology and control technology research of seawater ciliate disease has become a research hotspot at present. [0003] Accurate classification of ciliates is the basis for their life history research and disease prevention and control. Parasitic ciliates are usually highly flattened on both sides due to adaptation, with dense cilia on the body surface and no differentiation. The mouthparts are usually located on one side of the worm body. It is very difficult (Long Hong'an, 2008). [0004] Before the formation o...
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IPC IPC(8): G01N1/28
Inventor 荣小军李彬王岚廖梅杰张正王印庚陈贵平
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI