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Extraction method of bitter gourd polysaccharides

A kind of extraction method, the technology of bitter gourd polysaccharide, is applied in the extraction field of bitter gourd polysaccharide

Inactive Publication Date: 2013-02-20
QINGDAO ADVANCED MARINE MATERIAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of polysaccharides mainly include solvent extraction, biological extraction, enhanced extraction, etc., but the solvent extraction in the prior art is time-consuming, and the extraction rate not tall

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Dry bitter gourd powder is passed through a 40-mesh sieve and added to ethanol solution with a concentration of 70%. The mass ratio of dry bitter gourd powder to aqueous ethanol solution is 1:30. It is extracted by ultrasonic enhancement. The ultrasonic power is 200W, and the extraction time is 80min. Extraction twice, The extraction temperatures were 40°C and 60°C respectively, then centrifuged to obtain the supernatant and filtered, evaporated and concentrated to 1 / 5 of the original volume, precipitated with ethanol, filtered, and the filtrate was centrifuged to obtain the crude extract. The measured polysaccharide extraction rate was 5.4% .

Embodiment 2

[0017] Bitter melon dry powder is passed through a 40-mesh sieve, and added to a 70% ethanol solution. The mass ratio of bitter gourd dry powder to ethanol aqueous solution is 1:30. It is extracted by ultrasonic enhancement. The ultrasonic power is 200W, and the extraction time is 60min. Extraction 3 times , the extraction temperatures were 40°C, 50°C, and 60°C, and then centrifuged to obtain the supernatant and filtered, evaporated and concentrated to 1 / 5 of the original volume, precipitated with ethanol, filtered, and the filtrate was centrifuged to obtain the crude extract, which was measured for polysaccharide extraction. The rate is 6.2%.

Embodiment 3

[0019] Bitter melon dry powder is passed through a 40-mesh sieve and added to a 70% ethanol solution. The mass ratio of bitter gourd dry powder to ethanol aqueous solution is 1:20. It is extracted by ultrasonic enhancement. The ultrasonic power is 200W, and the extraction time is 60min. Extraction 3 times , the extraction temperatures were 40°C, 50°C, and 60°C, and then centrifuged to obtain the supernatant and filtered, evaporated and concentrated to 1 / 5 of the original volume, precipitated with ethanol, filtered, and the filtrate was centrifuged to obtain the crude extract, which was measured for polysaccharide extraction. The rate is 5.7%.

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Abstract

The invention provides an extraction method of bitter gourd polysaccharides. Dry bitter gourd powder is sieved with a 40-mesh sieve, sieved bitter gourd powder is added to ethanol water solution and is subjected to ultrasonic-assisted extraction, supernatant is obtained after centrifugation, is filtered, is evaporated and concentrated until the volume is 1 / 5 of the original volume, is precipitated by ethanol and is filtered, and the filtrate is centrifuged to obtain the crude extracts. The extraction method is characterized in that the ultrasonic-assisted extraction process is as follows: the ultrasonic power is 200W, the extraction time is 60-80 minutes, extraction is carried out twice to three times, and the extraction temperature is gradually increased. By adopting the extraction method, the extraction time can be shortened and the polysaccharide extraction rate can be improved, and the method has the characteristics of time saving and high efficiency.

Description

technical field [0001] The invention relates to a method for extracting bitter gourd polysaccharide, which belongs to the field of plant extraction. technical background [0002] Polysaccharides, referred to as polysaccharides, are polysaccharides formed by connecting more than 10 monosaccharide groups through glycosidic bonds. Polysaccharides are widely distributed and are essential components of all living organisms. Polysaccharides can not only be used as energy storage substances, but also have many biological activities. For example, polysaccharides can specifically inhibit the formation of tumor blood vessels, and induce apoptosis to inhibit tumors. It is harmless to normal cells and is superior to traditional surgical treatment and chemotherapy. . Because polysaccharides play an extremely important role in various biological growth processes, coupled with their unique physical, chemical and biological properties, polysaccharides have become a research hotspot in t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘连河
Owner QINGDAO ADVANCED MARINE MATERIAL TECH
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