Chemiluminescence immunoassay for helicobacter pylori in gastric mucosa sample

Abstract

The invention relates to a chemiluminescence immunoassay for helicobacter pylori in a gastric mucosa sample. The chemiluminescence immunoassay comprises the following steps of: (a), preparing a sample diluent with a test substance; (b), forming an antibody-antigen compound; (c), separating the sample obtained in the step (b) from the antibody-antigen compound obtained in the step (b); (d), forming an antibody-antigen-antibody compound; (e), adding a luminescence substrate; and (f), measuring the amount of the marked antibody, wherein the sample diluent contains protein and alkaline matter, and the pH of the sample diluent is 8.0-10. The chemiluminescence immunoassay for helicobacter pylori in the gastric mucosa sample provided by the invention ensures the accuracy of the test result through the sample diluent containing the protein and the alkali matter. Meanwhile, the luminescence substrate is low in cost, and the composite enhancer enables a light signal to be enhanced and everlasting.

Description

technical field [0001] The invention relates to a chemiluminescence immunoassay method for Helicobacter pylori in a gastric mucosa sample. Background technique [0002] Helicobacter pylori (H. pylori), a bacterium found in the upper gastrointestinal tract of humans, has been found to be associated with gastroduodenal disorders such as peptic ulcer, gastritis, and other diseases. The bacterium was classified by origin into the genus Campylobacter and then on the basis of more detailed information on its ultrastructure and fatty acid composition in the genus Helicobacter. [0003] There are many detection methods for Helicobacter pylori, the commonly used methods are: [0004] (1) Bacterial culture method. This method is the "gold standard" for diagnosing H. pylori. This method can directly prove the existence of H.pylori without false positive, and it can also be used for drug susceptibility test. Genetic factor studies on different strains. However, the technical condit...

AI Technical Summary

Problems solved by technology

However, it has been found that acid-suppressing drugs, some antibiotics, etc. will affect the urease test, and acid-suppressing drugs may be used for patients

(3) pH method, because there are false positives due to other mixed urease, visual errors in color judgment, etc.

Chemiluminescence immunoassay not only has the high sensitivity of radioimmunoassay, but also has the characteristics of simple and fast operation of enzyme-linked immunoassay, easy to standardize operation, and does not use harmful reagents in the test, and the reagents have a long retention period. In recent years, it has been clinically used in detection Various hormones, tumor markers, drugs and other trace biologically active substances, but due to the difficulty of sample sampling and other reasons, are rarely used in the detection of Helicobacter pylori in gastric mucosal samples, while the traditional detection of Helicobacter pylori in gastric mucosal samples Not very reliable, there are some false positives, the test results are inaccurate, and the cost is high