Tricholoma lobayense strain KJH-3 and preparation method thereof

A technology of Flammulina velutipes and strains, which is applied in the field of microorganisms, can solve the problems that the protection of Flammulina velutipes is not widely used, the development of Flammulina velutipes is restricted, and the requirements for production technology and technical elements are relatively high.

Inactive Publication Date: 2013-03-27
KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The deficiencies of the prior art are that its biological characteristics are still in the stage of experimental research, the biotransformation rate is relatively low, and it needs to be cultivated with clinker, and the requirements for production technology and technical elements are relatively high, thus restricting the development of Jinfu mushroom commodity economy
In addition, it can only be preliminarily determined whether it is a pure culture of Jinfu mushroom according to the shape of mycelia and isolates; the produced strains generally use solid strains
At present, the use of liquid strains for the protection of golden blessing mushroom resources is not widely used in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Inoculate the sporocarp tissue pieces of Jinfu Mushroom on the slant of the culture medium for separation and purification of the above strains, and cultivate them at 18°C ​​for 10 days. Observe and record the separated cultured test tubes every day, remove the contaminated test tubes in time, and select the non-polluted ones. , a strain with good growth potential, and obtained the isolated test tube species of Jinfu mushroom

[0035] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the mycelium block and inoculate it on the slant of the medium, and cultivate it at 18°C ​​for 7 days to obtain Jinfu mushroom Purified test tube species

[0036] 3. Using the test fruiting body and the corresponding mycelia isolates, extract the total DNA according to the SDS method respectively, carry out PCR amplification and ITS sequence determination, and compare the ITS seq...

Embodiment 2

[0041] The step of bacterial classification preparation is identical with embodiment one

[0042] 1. Inoculate the tissue pieces of the fruiting body of Jinfu mushroom on the slant of the above-mentioned strain separation and purification medium, and cultivate it at 22°C for 6 days. Observe and record the separated and cultured test tubes every day, remove the contaminated test tubes in time, and select the non-polluted ones. , a strain with good growth potential, and obtained the isolated test tube species of Jinfu mushroom

[0043] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the tip mycelium block and inoculate it on the slant of the medium, and cultivate it at 22°C for 6 days to obtain Jinfu Mushroom purification test tube species

[0044] 3. Using the test fruiting body and the corresponding mycelia isolates, extract the total DNA according to the SDS method...

Embodiment 3

[0049]1. Inoculate the tissue pieces of the fruiting body of Jinfu mushroom on the slant of the above-mentioned strain separation and purification medium, and cultivate it at 24°C for 5 days. Observe and record the separated and cultured test tubes every day, remove the contaminated test tubes in time, and select the non-polluted ones. , a strain with good growth potential, and obtained the isolated test tube species of Jinfu mushroom

[0050] 2. Inoculate the mycelium block of the isolated test tube on the plate culture dish of the above-mentioned agar medium to purify the strain. After 2 days, take the tip mycelium block and inoculate it on the slant of the medium, and cultivate it at 24°C for 5 days to obtain Jinfu Mushroom purification test tube species

[0051] 3. Using the test fruiting body and the corresponding mycelia isolates, extract the total DNA according to the SDS method respectively, carry out PCR amplification and ITS sequence determination, and compare the IT...

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Abstract

The invention relates to a selection and preparation method of tricholoma lobayense strains, belonging to the technical field of microorganisms. A strain tricholoma lobayense KJH-3 provided by the invention is preserved in the China general microbiological culture collection center with a preservation number of CGMCCNO.6703 on October 11, 2012. Excellent liquid or excellent solid cultures prepared from the strain are applied to the reproduction improvement of tricholoma lobayense so that obvious social and economic effects and a wide application prospect are realized.

Description

Technical field [0001] The invention is the field of microbial technology, which involves KJH-3 and preparation methods of Golden Mushroom strains. Background technique [0002] Globe Tricholoma lobayensc Also known as huge mouth mushrooms, big white mouth mushrooms, and Labari mushrooms, it is a new variety of rare edible fungus. There are small area cultivation in southern my country and Taiwan Province. [0003] In Jinfu mushroom cultivation technology, the acquisition of excellent bacteria is a key technical link.The obtaining Golden Fuel strain is usually used to separate and purify the cultivation of the body or spores, and is directly applied to the cultivation of the mushrooms or the production of mycelium.The lack of existing technology is that its biological characteristics are still in the experimental research stage, the biological conversion rate is low, and it must be cultivated with clinker. The production process and technical elements are high. ThereforeEssenceIn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H15/00A01G1/04C05G3/00
Inventor 罗孝坤张微思郭永红田永生张利菁
Owner KUNMING INST OF EDIBLE FUNGI CHINA NAT SUPPLY & MARKETING GENERAL COOP
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