Device and method for single-used matrix sampling
A sampling device and disposable technology, applied in the direction of sampling devices, biological material sampling methods, biochemical equipment and methods, etc., can solve the problems of unable to sample cell culture substrates and restricting large-scale production
Active Publication Date: 2013-03-27
CESCO BIOENGINEERING CO LTD
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Problems solved by technology
Traditional fixed-bed bioreactors cannot sample the cell culture substrate from the bioreactor system, and during cell culture procedures, especially in the production of human vaccines or therapeutic biopharmaceuticals, due to the need to Analyze cell properties that limit applications aimed at large-scale production
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[0054] The following detailed description is by way of example and is not intended to limit the invention to any particular described embodiment. These detailed descriptions can be further understood by reference to the accompanying drawings, which are hereby incorporated by reference. In order to avoid unnecessarily limiting the preceding description, the following description discusses the present invention in terms of certain preferred embodiments.
[0055] One embodiment of the present invention describes a sampling device for aseptically collecting cell culture substrates from a packed bed bioreactor. The disposable substrate sampling device of the present invention mainly includes two parts: one is a flexible tube; the other is a substrate collecting device. The flexible tube can be a thermoplastic tube, such as a C-Flex tube, or a high temperature resistant thermoplastic tube, such as a silicone tube. In one embodiment, the flexible tube is made of plastic film; or it...
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A single-used matrix sampling device for the single-used, disposable or autoclavble packed-bed bioreactor is provided herein. The present invention includes a tube means and a matrix collecting means. The matrix collecting means configured for collecting the cell culture matrix includes a receptacle and a pulling means connected to the receptacle or the matrix collecting means could be scoop-like in configuration; the tube means is configured for preventing the matrix collecting means from being contaminated while mechanically or pneumatically sampling; and the tube means could be sealed to enclose the sampled matrix collecting means after sampling. A single-used matrix sampling method for a single-used, disposable or autoclavble packed-bed bioreactor is also provided. The present invention could be utilized to aseptically collect the cell culture matrix from the packed-bed bioreactor without requiring to face the risks of contamination.
Description
[0001] Related Applications / Patents and Incorporated References [0002] The present application claims the priority of U.S. Provisional Patent Application 61 / 627,062, entitled "Matrix sampling device", filed on September 15, 2011, and it and the documents cited herein, or the documents cited by the cited documents, are referred to as documents way included. technical field [0003] The invention relates to a disposable matrix sampling technology, in particular to a disposable matrix sampling device and method, which are used for disposable, disposable or sterilizable reusable filled solidified bed bioreactors. Cell culture substrates were aseptically sampled. Background technique [0004] Single-use or disposable products are becoming more and more popular in the pharmaceutical industry due to the advantages of reducing cleaning validation steps, reducing costs and increasing product conversion rates. Especially for bioreactors used in animal or mammalian cell culture, it...
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IPC IPC(8): C12M1/26C12Q1/24
CPCG01N1/14C12Q1/02C12M33/04A61L2/07
Inventor 何玉书陈宥儒张景明
Owner CESCO BIOENGINEERING CO LTD