Infectious bursal disease VP2 gene and application thereof

A technology for bursal disease and chicken infectivity, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as immune failure

Active Publication Date: 2013-03-27
QINGDAO VLAND BIOTECH INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Vaccination is an effective measure to prevent infectious bursal disease. Although the attenuated vaccines and inactivated vaccines commonly used at present have played a considerable role in the prevention of IBD, due to the avian in

Method used

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  • Infectious bursal disease VP2 gene and application thereof
  • Infectious bursal disease VP2 gene and application thereof
  • Infectious bursal disease VP2 gene and application thereof

Examples

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Embodiment Construction

[0010] The main structural protein VP2 protein of IBDV is the most important host protective antigen. The present invention amplifies the super-virulent VP2 gene of IBD by PCR method, clones it on the pET-28a vector, transforms BL21(DE3), and induces it with IPTG After the VP2 protein is expressed, the expressed bacterium is homogenized under high pressure and then centrifuged to obtain the supernatant to obtain the VP2 protein solution, which is then added with mineral oil adjuvant to make an oil emulsion vaccine, which can be used to prevent chicken infectious bursal disease.

[0011] The chicken infectious bursal virus VP2 gene of the present invention will be described in detail below in conjunction with specific examples.

[0012] 1. Screening of VP2 gene

[0013] A pair of primers for amplifying chicken infectious bursal disease virus VP2 whole gene were designed with Primer5.0 software, and a protective base and a BamH I restriction site were added to the 5' end of the ...

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Abstract

The invention relates to an infectious bursal disease VP2 gene, wherein the amino acid sequence of the gene is SEQ ID NO: 1. The infectious bursal disease VP2 gene sieved by the invention is used as a subunit vaccine in genetic engineering. The subunit vaccine prepared by the infectious bursal disease VP2 gene sieved by the invention makes SPF chickens which are 21 days old immune. After 21 days, chickens are of strong immunity, so that the immune chickens can resist the attack of virulent virus of infectious bursal disease.

Description

technical field [0001] The invention belongs to the technical field of poultry antigen screening, and in particular relates to a chicken infectious bursal disease VP2 gene and application thereof. Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD), also known as chicken infectious supracoelomal bursal disease, is an acute, contagious disease caused by infectious bursal virus. It is characterized by inflammation, necrosis, atrophy and severe damage of lymphocytes in the bursa of Fabricius, which can cause immune dysfunction in chickens and interfere with the immune effects of various vaccines. The disease has a high incidence and is currently one of the most important diseases in the poultry industry. [0003] Vaccination is an effective measure to prevent infectious bursal disease. Although the attenuated vaccines and inactivated vaccines commonly used at present have played a considerable role in the prevention of IBD, due to t...

Claims

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Application Information

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IPC IPC(8): C12N15/40C07K14/08A61K39/12A61P31/14
Inventor 王雷凌红丽王睿智张园园
Owner QINGDAO VLAND BIOTECH INC
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