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Treatment of diabetes with pancreatic endocrine precursor cells

A precursor cell, endocrine technology, applied in pancreatic cells, embryonic cells, animal cells, etc., can solve the problem of not completely simulating the developmental procedures of higher mammals

Active Publication Date: 2013-04-17
JANSSEN BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] However, mouse models of embryonic stem cell development may not fully mimic the developmental program in higher mammals such as humans

Method used

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  • Treatment of diabetes with pancreatic endocrine precursor cells
  • Treatment of diabetes with pancreatic endocrine precursor cells
  • Treatment of diabetes with pancreatic endocrine precursor cells

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Experimental program
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Effect test

example

[0193] Cells of the human embryonic stem cell line H1 were cultured (1:30 dilution) on MATRIGEL-coated plates and differentiated into pancreatic endocrine precursors using the following protocol:

[0194] a. Add 20ng / ml WNT-3a (catalogue number: 1324-WN-002, R&D Systems, MN) plus 8ng / ml of bFGF (catalogue number: 100-18B, PeproTech, NJ) in RPMI medium (catalogue number 22400, Invitrogen, Ca) and cultured for one day, then supplemented with 2% BSA and 100ng / ml The RPMI medium of Activin A plus 8ng / ml bFGF was treated for another two days (phase 1), and then

[0195] b. Treat three days (phase 2) with DMEM / F12 (Catalogue No. 11330, Invitrogen, Ca)+2%BSA+50ng / ml FGF7, then

[0196] c. Supplemented with 1% B27 (No. 17504-044, Invitrogen, CA) + 50 ng / ml FGF7 + 0.25 μM cyclopamine-KAAD (No. 239804, Calbiochem, CA) + 2 μM retinoic acid as shown in Table 1 (RA) (Sigma, MO)+100ng / ml Noggin (R&D Systems, MN) was cultured for four days in different basal media (Stage3), and then

[01...

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Abstract

The present invention provides a method for lowering blood glucose levels in an animal by transplanting a population of pancreatic endocrine precursor cells into an animal.

Description

[0001] Cross references to related patent applications [0002] This application claims the benefit of US Provisional Patent Application Serial No. 61 / 373,109, filed August 12, 2010, which is hereby incorporated by reference in its entirety for any purpose. technical field [0003] The present invention provides a method of reducing blood glucose levels in an animal by transplanting a population of pancreatic endocrine precursor cells into the animal. Background technique [0004] Advances in cell replacement therapy for type I diabetes and the scarcity of transplantable islets have focused attention on developing a source of insulin-producing cells or beta cells suitable for transplantation. One approach is to generate functional beta cells from pluripotent stem cells, such as embryonic stem cells. [0005] In vertebrate embryonic development, pluripotent cells can give rise to a cell population comprising the three germ layers (ectoderm, mesoderm and endoderm) in a proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K38/18A61K38/22A61P3/10A61K35/39
CPCA61K35/12A61K35/39C12N5/0676C12N2501/115C12N2501/155C12N2501/16C12N2501/415C12N2501/727C12N2506/02C12N5/0012A61P3/00A61P3/10A61K9/48A61K38/10A61K38/18
Inventor J.徐
Owner JANSSEN BIOTECH INC
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