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Production process of composite probiotics

A technology of compound probiotics and production process, applied in application, animal feed, animal feed, etc., can solve the problems of short shelf life, evolution of probiotic population structure, low biological activity, etc., to achieve good antibacterial effect, prevent pollution, good antibacterial effect

Active Publication Date: 2014-02-12
GUANGZHOU PROFORCARE PHARMA INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the research, development and application of compound probiotics in my country is still in its infancy, and the production methods of most compound probiotics are only simple expansion of laboratory preparation methods, which are not suitable for large-scale and continuous industrial production, and are not suitable for temperature , The control of fermentation time is not strict enough, resulting in the problems of short shelf life, evolution of probiotic population structure, and low biological activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, concrete processing steps are as follows:

[0058] (1) Breeding:

[0059] ① Add 700g barley malt, 600g peptone, 1000g agar, 300g sodium citrate, 150g potassium dihydrogen phosphate, 7.5g magnesium sulfate, 200g sodium bicarbonate into the batching tank, make up to 100L with distilled water, and adjust the pH value after heating to boiling Transfer to the saccharification tank after reaching 4.5, add 2.1×10 5 U saccharification enzyme, saccharification at 60°C for 2 hours to obtain 104.6kg saccharification liquid;

[0060] ②Transfer 104.6kg of saccharification liquid into the fluid heater, raise the temperature to 95°C within 5s, keep it warm for 1h, and sterilize at high temperature. Culture medium;

[0061] ③ Distribute 103.9 kg of breeding medium equally to four chemostats, and use the arrow-type inoculator to inoculate the strains of lactic acid bacteria, bifidobacteria, bacillus subtilis, and candida utilis cultivated in the laboratory by 2% respec...

Embodiment 2

[0071] Embodiment 2, concrete processing steps are as follows:

[0072] (1) Breeding:

[0073] ① Add 2.4kg barley malt, 1.6kg peptone, 2.4kg agar, 0.8kg sodium citrate, 0.36kg potassium dihydrogen phosphate, 0.019kg magnesium sulfate, 0.6kg sodium bicarbonate into the batching tank, make up to 200L with distilled water, and heat to After boiling, adjust the pH value to 5.0 and transfer to the saccharification tank, add 9.6×10 5 U saccharification enzyme, saccharification at 60°C for 4 hours to obtain 212.44kg saccharification liquid;

[0074] ②Transfer 212.44kg of saccharification liquid into the fluid heater, raise the temperature to 100°C within 8s, keep it warm for 1.5h, and sterilize at high temperature. breeding medium;

[0075] ③Distribute the 210.18kg breeding medium equally to four chemostats, and use the arrow type inoculator to inoculate the strains of lactic acid bacteria, bifidobacterium, bacillus subtilis and candida utilis cultivated in the laboratory by 4% r...

Embodiment 3

[0085] Embodiment 3, concrete processing steps are as follows:

[0086] (1) Breeding:

[0087] ① Add 2.85kg of barley malt, 2.1kg of peptone, 3.3kg of agar, 1.05kg of sodium citrate, 0.495kg of potassium dihydrogen phosphate, 0.0255kg of magnesium sulfate, and 0.75kg of sodium bicarbonate into the batching tank, make up to 300L with distilled water, and heat to After boiling, adjust the pH value to 4.8 and transfer to the saccharification tank, add 9.98×10 5 U saccharification enzyme, saccharification at 60°C for 3 hours to obtain 311.9kg saccharification liquid;

[0088] ②Transfer 311.9kg of saccharification liquid into the fluid heater, raise the temperature to 98°C within 6s, keep it warm for 1.3h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and cools down to 35°C within 7s to obtain 310.08kg breeding medium;

[0089] ③ Distribute 310.08 kg of breeding medium equally to four chemostats, and use an arrow-type i...

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Abstract

The invention discloses a production process of composite probiotics. The process comprises the following specific steps of: 1, breeding, namely (1) preparing a culture solution, and heating, dissolving and saccharifying the culture solution to obtain a saccharified solution; (2) sterilizing the saccharified solution at a high temperature, and cooling the sterilized saccharified solution to obtain a breeding culture medium; and (3) inoculating strains of lactic acid bacteria, bifidobacterium, bacillus subtilis and candida utilis into the breeding culture medium for culturing, thus obtaining four mature bacterium solutions; 2, fermentation, namely (1) mixing and sterilizing bean pulp and bran, thus obtaining a solid material; (2) respectively adding the four mature bacterium solutions into a diluent, thus obtaining four diluted bacterium solutions after diluting; and (3) respectively inoculating strains of the four diluted bacterium solutions into the solid material for fermentation, thus obtaining four fermented materials; and 3, drying and packaging, namely (1) mixing the four fermented materials and drying the mixed materials for the first time; (2) drying the mixed materials for the second time; (3) drying the mixed materials for the third time; and (4) crushing, outputting and packaging the dried materials to obtain the composite probiotics. The process can realize large-scale and continuous production and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a production process of compound probiotics. Background technique [0002] With the rapid development of the current feed industry, various feed additives are also showing a trend of diversification. Due to the gradual emergence of various disadvantages of antibiotics, compound probiotics can adjust the structure of animal intestinal flora, improve the activity of digestive enzymes in animals, improve The excellent characteristics of animal production performance and improving animal immunity have gradually become an indispensable feed additive component in animal husbandry. [0003] At present, the research, development and application of compound probiotics in my country is still in its infancy, and the production methods of most compound probiotics are only simple expansion of laboratory preparation methods, which are not suitable for large-scale and continuous industr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00
Inventor 李晓叶
Owner GUANGZHOU PROFORCARE PHARMA INC
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