Kit for detecting anaplasma bovis

Abstract

The invention discloses a kit for detecting anaplasma bovis. The kit for detecting anaplasma bovis at least contains the following three primer probe sequences: a forward primer SEQ No.1, a reverse primer SEQ No.2 and a probe SEQ No.3, wherein a fluorescent luminophore FAM is combined to a fifth end of the probe sequence, and a non-fluorescent quencher connected with an MGB (minor groove binder) is combined to a third end of the probe sequence. The application of the kit provides a real-time fluorescent quantitative PCR (polymerase chain reaction) method for detecting anaplasma bovis by using a 16srRNA gene. The kit has good sensitivity, specificity and repeatability, and the sensitivity of the kit is more than 10 times than that of nested PCR, so that the kit can be used for quick quantitative detection of the anaplasma bovis.

Description

technical field [0001] The invention relates to a kit for detecting pathogenic bacteria in animal blood, more precisely, the invention relates to a kit for detecting bovine anemia. Background technique [0002] Anaplasma is a type of obligate intracellular parasite transmitted by ticks. It mainly parasitizes in the blood cells of ruminants such as cattle and sheep. The disease it causes is called anaplasmosis. Bovine anplasma disease was first discovered in North Africa in 1910 and later found to be widely distributed throughout the world. Anaplasma disease causes animal weight loss, miscarriage, decreased milk production, and severe cases lead to death, resulting in serious economic losses. In view of this, the World Organization for Animal Health (OIE) listed it as a disease that must be notified. The pathogens that cause bovine anplasma disease have been confirmed as anaplasma marginale, anaplasma centrale, anaplasma bovis and anaplasma phagocytophilum. [0003] Anapla...

AI Technical Summary

Problems solved by technology

Blood smear staining method is difficult to detect the pathogen under the microscope when the infection rate is very low or in the early stage of infection, and the proficiency of the operation will directly affect the accuracy of the diagnosis result

Nested PCR detection method, see: Kawahara M, et al. 2006, Novel genetic

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