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Immunohistochemical quality control reference object and quality control method

A technology of immunohistochemistry and reference materials, which is applied in the direction of biological testing, measuring devices, and preparation of test samples. It can solve the problems of providing and difficult to detect tissues, and achieve consistent quality control signals, long-term stability, and low cost. Effect

Active Publication Date: 2015-04-15
FUZHOU MAIXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the positive and negative control tissue methods currently used have certain effects, it is difficult to provide a standard content reference for the detection tissue because each tissue slice has certain differences.
And there is currently no non-biological tissue material on the market as a control material for immunohistochemical quality control

Method used

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  • Immunohistochemical quality control reference object and quality control method

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preparation example Construction

[0031] The preparation steps of the negative control material in the present invention are as follows: Weigh 0.2g of skimmed milk powder, dissolve it in 10mL of double distilled water, and fully stir to dissolve. Weigh 0.2 g of dextran and add it to the above solution, after stirring, add 20 μL of glacial acetic acid, stir continuously to dissolve the dextran completely, and place it at 4°C to remove the air bubbles in the dextran solution. Beat the dextran solution into the sodium hydroxide solution with a disposable injection needle to solidify, forming white solid particles. The obtained solid particles were subjected to the same procedures as pathological tissues, dehydrated with gradient alcohol (dehydration steps: 80%, 90%, 95%, 100% ethanol with various concentrations were dehydrated for 2 hours), transparent with xylene, soaked in paraffin , and finally made into paraffin specimens by paraffin embedding. Slice and mount according to the same procedure as pathological ...

Embodiment 1

[0035] Embodiment 1, the preparation of negative control material

[0036] Weigh 0.2g of skimmed milk powder, dissolve in 10mL of double distilled water, stir well to dissolve. Weigh 0.2 g of dextran and add it to the above solution, after stirring, add 20 μL of glacial acetic acid, stir continuously to dissolve the dextran completely, and place it at 4°C to remove the air bubbles in the dextran solution. Beat the dextran solution into the sodium hydroxide solution with a disposable injection needle to solidify, forming white solid particles. The obtained solid particles were subjected to the same procedures as pathological tissues, dehydrated with gradient alcohol (dehydration steps: 80%, 90%, 95%, 100% ethanol with various concentrations were dehydrated for 2 hours), transparent with xylene, soaked in paraffin , and finally made into paraffin specimens by paraffin embedding. Slice and mount according to the same procedure as pathological tissue.

Embodiment 2

[0037] Embodiment 2, the preparation of positive control material

[0038] Measure 10 mL of double distilled water, add antigen protein with a final concentration of 0.1 mg / L and fully dissolve it. Weigh 0.2g of skimmed milk powder, dissolve it in the above antigen solution, stir well to dissolve. Weigh 0.2 g of dextran and add it to the above solution, after stirring, add 20 μL of glacial acetic acid, stir continuously to dissolve the dextran completely, and place it at 4°C to remove the air bubbles in the dextran solution. Beat the dextran solution into the sodium hydroxide solution with a disposable injection needle to solidify, forming white solid particles. The obtained solid particles are dehydrated by gradient alcohol (dehydration steps: 80%, 90%, 95%, 100% ethanol with various concentrations are dehydrated for 2 hours respectively), transparent in xylene, soaked in paraffin, embedded in paraffin into paraffin specimens.

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Abstract

Provided in the present invention are an immunohistochemical quality control reference object and a method for quality control using the reference object. In the present invention, particles solidified by a glucan solution are used to simulate biological tissue, and target antigen proteins are uniformly embedded in the particles. The solid glucan particles in which the target antigens are embedded are formed into a paraffin block after being dehydrated, transparentized, wax-dipped and paraffin-embedded, and the paraffin block is stuck to a glass slide after being sliced on a slicer, so as to be dyed together with tissue to be detected. In the method of the present invention, negative control, positive control and antigen retrieval control are designed according to different materials, and the purpose of quality control is achieved through a dyeing result of a reference object.

Description

technical field [0001] The invention belongs to the field of biopathological detection. Specifically, the invention relates to an immunohistochemical quality control reference substance and a quality control method using the reference substance. Background technique [0002] In order to promote the standardization of immunohistochemical laboratories and to detect the reliability of immunohistochemical staining results, the immunohistochemical process should be subject to effective quality control. Immunohistochemical quality control refers to the adoption of continuously optimized measures and technologies to formulate laboratory systems and routine work, standardize and improve laboratory immunohistochemical staining procedures and procedures, and ensure the best quality of immunohistochemical staining. An accurate diagnosis can be made. [0003] Immunohistochemical staining is a very important technique and means in clinical pathological diagnosis and morphological resear...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53G01N33/531
CPCG01N1/06G01N1/28G01N2001/2893G01N33/531G01N33/54386G01N2001/368
Inventor 林齐心熊玉林王小亚
Owner FUZHOU MAIXIN BIOTECH CO LTD
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