Flemingia philippinensis total flavonoids and beta-cyclodextrin clathrate compound and preparation method thereof

A technology of β-cyclodextrin and total flavonoids, which is applied in the field of pharmaceutical processing to achieve the effects of high inclusion rate, improved bioavailability, and increased solubility

Inactive Publication Date: 2013-06-12
JILIN INST OF CHEM TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Through literature search, there is no report on the preparation of t

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preparation example Construction

[0023] 1. Preparation of total flavonoids

[0024] Weigh an appropriate amount of medicinal material, grind it, and extract it twice with ethanol solution under reflux, 2 hours each time, combine the filtrate and concentrate to obtain the extract; add water to dissolve, centrifuge, and take the processed AB-8 macroporous resin on the supernatant The column was eluted with water and 10% ethanol in sequence to remove impurities, and then eluted with 70% ethanol solution, the eluted part was collected, and the solvent was recovered to obtain the total flavonoids with a purity of 65%.

[0025] 2. Preparation of total flavonoid β-cyclodextrin inclusion complex

Embodiment 1

[0026] Example 1: Using the saturated aqueous solution method, accurately weigh an appropriate amount of β-cyclodextrin, add distilled water, heat and dissolve in a boiling water bath, cool at room temperature, and stir magnetically for 30 minutes; accurately weigh the above-mentioned total flavonoids, add a small amount of absolute ethanol to dissolve, Slowly add to the β-cyclodextrin inclusion solution, the molar ratio of total flavonoids to β-cyclodextrin is 1:1, stir for 0.5 hours, refrigerate at 4°C for 24 hours, filter under reduced pressure, and wash the filter cake with a small amount of absolute ethanol , The filter cake was vacuum-dried to constant weight at 60°C to obtain the total flavonoid β-cyclodextrin inclusion complex with an inclusion rate of 61%.

Embodiment 2

[0027] Example 2: Using the saturated aqueous solution method, accurately weigh an appropriate amount of β-cyclodextrin, add distilled water, heat and dissolve in a boiling water bath, cool at room temperature, and stir magnetically for 30 minutes; accurately weigh the above-mentioned total flavonoids, add a small amount of absolute ethanol to dissolve, Slowly add to the β-cyclodextrin inclusion solution, the molar ratio of total flavonoids and β-cyclodextrin is 1:2, stir for 1 hour, refrigerate at 4°C for 24 hours, filter under reduced pressure, and wash the filter cake with a small amount of absolute ethanol , and the filter cake was vacuum-dried at 60°C to constant weight to obtain the inclusion complex of total flavonoids β-cyclodextrin with an inclusion rate of 68%.

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Abstract

The invention relates to a flemingia philippinensis total flavonoids and beta-cyclodextrin clathrate compound. The flemingia philippinensis total flavonoids and beta-cyclodextrin clathrate compound contains the active components including flemingia philippinensis total flavonoids and beta-cyclodextrin of an inclusion agent. The preparation method of the flemingia philippinensis total flavonoids comprises the following steps of: refluxing and extracting with ethanol by taking flemingia philippinensis as a raw material; carrying out adsorption separation on an extract extractum through AB-8 macroporous resin to obtain flemingia philippinensis total flavonoids; the preparation method of the clathrate compound comprises the following steps of: preparing a saturated aqueous solution from beta-cyclodextrin by using a saturated aqueous solution method; adding the dissolved flemingia philippinensis total flavonoids into a beta-cyclodextrin saturated solution according to a proper adding ratio of flemingia philippinensis total flavonoids to beta-cyclodextrin; stirring; refrigerating; carrying out extraction filtration, drying; grinding; and sieving to obtain the flemingia philippinensis total flavonoids and beta-cyclodextrin clathrate compound. By using the clathrate compound provided by the invention, the dissolubility and the bioavailability of the flemingia philippinensis total flavonoids can be improved.

Description

technical field [0001] The invention relates to a beta-cyclodextrin inclusion compound of total flavonoids of jinjinba and a preparation method of the inclusion compound, belonging to the technical field of pharmaceutical processing. Background technique [0002] Qianjinba belongs to the subfamily Papilionoideae of Leguminosac, and is widely distributed in the southeast, south-central, and southwest regions of China. The root of Qianjinba is used as medicine, which has the effect of dispelling rheumatism and strengthening the waist and knees. It is used for rheumatoid arthritis, lumbago and leg pain, lumbar muscle strain, and bruises. Qianjinba contains a large amount of active substances, among which flavonoids have the functions of antibacterial, anti-inflammatory, anti-oxidation, lowering blood fat, reducing blood clots, preventing vascular sclerosis and anti-aging. However, it has poor water solubility and cannot be well absorbed by the human body. [0003] β-cyclodext...

Claims

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Application Information

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IPC IPC(8): A61K36/48A61K47/40A61P31/04A61P29/00A61P39/06A61P3/06A61P7/02A61P9/00
Inventor 陈帅朱琳王慧竹薛健飞霍春阳姜立欣
Owner JILIN INST OF CHEM TECH
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