Method for extracting polyphenol from camellia

A technology of camellia and flowers, applied in the field of molecular biology, can solve the problems of unreported, waste, waste of resources, etc.

Inactive Publication Date: 2013-06-12
YIBIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many studies have shown that polyphenols have been successfully extracted from the leaves of Camellia japonica, there has been no report on the extraction of polyphenols from its flowers, and the flowers of Camellia japonica are not used and wasted every year due to falling.
[0003] Shortcomings of the prior art: there is no research on extracting polyphenols from camellia flowers at present. As an ornamental plant, camellia has sufficient resources, but has not been effectively utilized, resulting in a waste of resources

Method used

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  • Method for extracting polyphenol from camellia
  • Method for extracting polyphenol from camellia
  • Method for extracting polyphenol from camellia

Examples

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Embodiment 1

[0055] A method for extracting polyphenols from camellia flowers, comprising the following steps:

[0056] (1) Preparation of camellia powder: pick the camellias that are in full bloom, wash them, store them in the refrigerator, freeze-dry them with a freeze dryer, pass them through a 18-mesh sieve after grinding, and finally seal them and store them at -20°C;

[0057] (2) Preparation of extract: Weigh 1.000g of camellia powder, add 35ml of ethanol with a volume concentration of 60%, put it in a water bath at 80°C for 20min, and filter; Ethanol was distilled off under reduced pressure, and then diluted with distilled water to a concentration of 2.5 mg.mL -1 , and then pass through a 0.45um filter membrane to obtain an extract;

[0058] (3) DM301 macroporous resin adsorption:

[0059] a: Weigh 20.0g macroporous resin, soak DM301 macroporous resin in ethanol for 4 hours, then let it go, use the same method to repeatedly soak and clean DM301 macroporous resin, until adding 3 ti...

Embodiment 2

[0065] A method for extracting polyphenols from camellia flowers, comprising the following steps:

[0066] (1) Preparation of camellia powder: pick the camellias that are in full bloom, wash them, store them in the refrigerator, freeze-dry them with a freeze dryer, pass them through a 18-mesh sieve after grinding, and finally seal them and store them at -20°C;

[0067] (2) Preparation of extract: Weigh 5.000g of camellia powder, add 175ml of 60% ethanol, put it in a water bath at 80°C for 20min, and then filter; depressurize the obtained filtrate at 35°C and 0.09KPa Distill to remove ethanol, then dilute with distilled water to a concentration of 2.50mg.mL -1 , and then pass through a 0.45um filter membrane to obtain an extract;

[0068] (3) DM301 macroporous resin adsorption:

[0069] a: Weigh 20.0g of macroporous resin, soak DM301 macroporous resin in ethanol for 3 hours, then let it go, soak and clean DM301 macroporous resin repeatedly in the same way, until adding 3 time...

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Abstract

The invention discloses a method for extracting polyphenol from camellia. The method is characterized by comprising the following steps of (1) preparation of camellia powder; (2) preparation of leach liquor; and (3) adsorption of resin. The method has the characteristics of simple preparation method, convenience in operation and low cost and the like, and can extract the high purity polyphenol from the camellia resource, and according to the method, the resource can be utilized reasonably, and the research target of turning waste into wealth can be met.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to a method for extracting polyphenols from camellia flowers. Background technique [0002] Polyphenols in plants are compounds with multiple phenolic groups, often including flavanols, anthocyanins, flavonoids, flavonols and phenolic acids. At present, the most studied natural polyphenols are tea polyphenols, which are flavanols obtained from edible tea leaves. Many studies have shown that they are a kind of natural antioxidants, which can scavenge free radicals, prevent lipid peroxidation, improve It has biological effects such as body immunity and anti-aging; it is also reported that it also has the effect of anti-radiation and inhibiting the proliferation of cancer cells. For this reason, tea polyphenols are developed to be used in food preservation and antisepsis, prolonging the shelf life of food, protecting the nutritional components in food, and have no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07G99/00
Inventor 罗通
Owner YIBIN UNIV
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