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Method for preparing cell chip

A cell chip and cell suspension technology, applied in the field of biotechnology applications, can solve the problems of high cost, unfavorable popularization of cell chips, complicated operation, etc., and achieve the effects of flexible use, low cost and easy operation.

Inactive Publication Date: 2013-06-19
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is complicated to operate and high in cost, which is not conducive to the widespread promotion of cell chips.

Method used

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  • Method for preparing cell chip
  • Method for preparing cell chip
  • Method for preparing cell chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0025] The structure of the biological sampling pen is as follows: figure 1 As shown, it consists of 6 parts. The tip 1 mainly provides the support point for the ball 3. The pen holder 2 is used to store the cell suspension. When the ball 3 rubs and rotates on the substrate, it provides sufficient shear force to drive the cell suspension 5 to flow out. , the connection device 4 is used to connect the pen holder 2 and the pen tip 1, and the sealing oil 6 prevents the sample (cell suspension) from volatilizing. When the bio-sampling pen writes on the substrate (similar to the writing process of a ballpoint pen or gel pen), the ball 3 at the front end of the pen head 1 rotates, driving the cell suspension 5 to flow out.

[0026] The cell chip is prepared by using a biological sample pen, including the following steps:

[0027] Step 1: Pour an appropriate amount of cell suspension (10μL-2mL) into the pen holder. The type and concentration of cells are determined by demand, and th...

example 1

[0032] Example 1 Preparation of breast cancer cell chip with biological sample pen

[0033] Step 1: Inject 1000 μL of breast cancer cell suspension into the pen holder, then seal it with mineral oil to prevent evaporation.

[0034] Step 2: Hold this biological sample pen to write a line on the glass plate ( figure 2 ).

[0035] Step 3: place the glass plate in an incubator for cultivation, maintain a humid environment at 37° C., and place a large amount of water around it to prevent evaporation.

[0036] Step 4: After 3h, the cells are completely attached to the wall ( image 3 ), add a large amount of medium to meet the needs of further cell growth.

[0037] Step 5: After 5 days, breast cancer cells grow into threads ( Figure 4 ), the results show that this method can be used to prepare cell chips, with flexible use and low cost.

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Abstract

The invention provides a method for preparing a cell ship. The method comprises the following steps of 1, pouring a cell suspension into a penholder of a sampling pen, wherein the sampling pen comprises a pen head; a front end of the pen head is provided with a sample outlet hole; a rotatable ball is arranged in the sample outlet hole; and a rear end of the pen head is communicated with the penholder, and then sealing the cell suspension in the penholder by sealing oil, 2, writing the cell suspension on a base by the sampling pen, and 3, putting the base with the cell suspension into a culture tank, and after cells adhere to the wall of the culture tank, carrying out cell culture to obtain a cell chip. The cell suspension is written on the base by the sampling pen and after the cells adhere to the wall of the culture tank, the cells are cultured and grow so that the cell chip is obtained. The method realizes cell chip quantitative micropreparation and has the characteristics of low cost, simple structure, simple and easy operation, flexible use and good controllability.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and in particular relates to a method for preparing a cell chip. Background technique [0002] Cell chip is a new concept proposed internationally in recent years. It refers to the growth of various living cells on the chip, so that it has the phenotype and function of internal organs, and the response of cells in the body to external stimuli is simulated on the chip. This chip will have important applications in drug screening, environmental monitoring, and anti-terrorism. The difficulty in making such a chip lies in how to precisely locate different living cells to a specific position on the chip to grow. At present, the method of microfluidic chips is mainly used in the world to transport living cells to specific locations. However, this method is complicated to operate and high in cost, which is not conducive to the widespread promotion of cell chips. Therefore, proposing a low-cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B50/06
Inventor 徐峰韩玉龙王琳卢天健
Owner XI AN JIAOTONG UNIV