Device and method for preparing and collecting lipidosome

A collection device and liposome technology, applied in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve problems such as increased difficulty, complicated operation, and increased investment, so as to improve separation efficiency and collection Efficiency, improve preparation efficiency, reduce the effect of intermediate process

Inactive Publication Date: 2013-07-03
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In summary, there are still some problems to be solved in the existing electro-preparation methods that use the chip structure to apply an electric field to prepare giant liposomes. The chips used in the early electro-preparation methods are simple and easy to process. However, the electro-preparation process It cannot be precisely controlled, and the improved chip proposed later can control the preparation process more precisely, but there are disadvantages such as difficult to observe, expensive, and complicated to operate.
In addition, all the chips that have been proposed do not include giant liposome sorting and collection parts. The sorting and collection of liposomes need to increase the post-processing steps, which increases the difficulty and investment, and affects the quality of giant liposomes. Sorting effect and final collection rate

Method used

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  • Device and method for preparing and collecting lipidosome
  • Device and method for preparing and collecting lipidosome
  • Device and method for preparing and collecting lipidosome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The liposome preparation and collection device of the present embodiment such as figure 1 , image 3 , Figure 3A , Figure 4 , Figure 4A , Figure 5 , Figure 5A , Image 6 , Figure 6A , Figure 7 with Figure 7A As shown, the device includes: a signal generator 1 for sending electrical signals, and a chip 2 for preparing and collecting liposomes. In this embodiment, the signal generator and the chip for preparing and collecting liposomes are connected by conductive tape 6 . A preparation chamber 3 , a collection chamber 4 , and a waste liquid chamber 5 are made in the chip 2 for preparing and collecting liposomes. The chip 2 is composed of a base plane and a wall material layer thereon, and the wall material layer is further divided into upper and lower layers.

[0036] The preparation chamber 3 includes a base electrode 7 and an upper layer electrode 10. The middle preparation chamber 8 is between the electrodes. The electrodes are connected to the signal...

Embodiment 2

[0042] The liposome preparation and collection device of the present embodiment such as figure 1 shown.

[0043] Mix and dissolve lecithin and cholesterol in ether solvent at a mass ratio of 5:1, add fluorescent dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) to stain the organic phase Treatment, then, the organic phase is added dropwise on the base electrode 7 of the preparation chamber, then, nitrogen is blown for 5 minutes to allow the ether to evaporate, and then the chip 2 for preparing and collecting liposomes is placed in a vacuum environment for 2 hours, and the ether is allowed to evaporate. Complete evaporation, after forming a dry lipid film on the base electrode, add a solution containing 200mM glucose and 20mM Nacl to the preparation chamber of the preparation chamber 3, and then connect the signal generator 1 and the upper layer electrode 10, the base electrode through the conductive tape 6 7. Apply an electrical signal to the prepar...

Embodiment 3

[0046] The structure of the liposome preparation and collection device used in this embodiment is as follows: figure 2 As shown, its basic structure is the same as that of Example 1, except that there are four collection chambers 4 and four waste liquid chambers 5, which are connected one to one to collect giant liposomes of different particle sizes.

[0047] Mix and dissolve lecithin and cholesterol in ether solvent at a mass ratio of 5:1, add fluorescent dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) to stain the organic phase Treatment, then, the organic phase is added dropwise on the base electrode 7 of the preparation chamber, then, nitrogen is blown for 5 minutes to allow the ether to evaporate, and then the chip 2 for preparing and collecting liposomes is placed in a vacuum environment for 2 hours, and the ether is allowed to evaporate. After complete evaporation and a dry lipid film formed on the base electrode, a solution containing micr...

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Abstract

The invention discloses a device and a method for preparing and collecting lipidosome. The device comprises a signal generator and a preparation and collection chip, wherein the preparation and collection chip comprises a preparation chamber, a collection chamber and a waste fluid chamber; the preparation chamber comprises an electrode couple and a preparation cavity; the electrode couple is connected with the signal generator through electric conduction tapes; the collection chamber comprises an upper-layer collection cavity and a lower-layer collection cavity; a layer of microfiltration membrane is arranged between the two cavities; and the preparation chamber, the collection chamber and the waste fluid chamber are connected through microchannels. The device for preparing and collecting lipidosome mainly uses the method of powering up to prepare the lipidosome, and utilizes the microfiltration membrane to collect and process the lipidosome on the same chip. Compared with the prior art, the device and the method have the following technical effects: the large blank space lipidosome and the large lipidosome carried with drug, protein and particles can be effectively prepared; the preparation and collection of the lipidosome are integrated to reduce pilot process; and the required dose of reagents is less.

Description

Technical field [0001] The present invention involves the field of lipid system preparation, which specializes in a lipid system preparation and collection device, as well as methods to prepare and collect lipids with the device. Background technique [0002] The liposomal is a phospholipid bipolar membrane assembled in the water phase to be closed in the water phase.Liposukes have a two -e -friend, biocompatibility, and similar structures as cell membrane and cellular membranes. They have been widely used in research fields such as beauty, food, medicine, and biochemistry.The particle size range of the liposomes is from 10nm to 100 μm. Among them, liposomes with a particle size greater than 5 μm are called giant lipids.Giant liposomes can be used as a vector for drug transmission, or it can be used as a micro -reactor to simulate cells or cells, and study biochemical reactions in the body. In addition, giant lipids can also be used for the simulation of cell membranes, performin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00
Inventor 杨军王振宇胡宁郑小林陈曦林炳文王琼
Owner CHONGQING UNIV
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