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Method for extracting diethylstilbestrol, hexestrol, dienestrol and/or bisphenol A from animal muscle

A technology of animal muscle and diethylstilbestrol, which is applied in the field of extracting diethylstilbestrol, diethylstilbestrol and/or bisphenol A, can solve the problems of high detection limit, achieve low detection limit, reduce extraction cost, and purify thorough effect

Active Publication Date: 2015-01-28
NINGBO ACAD OF OCEAN & FISHERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Only the external standard method can be used to determine diethylstilbestrol, hexestrol, dienestrol, etc., and the detection limit is relatively high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Take two samples of 5.00 g of shrimp meat without artificial synthetic hormones, chop and homogenize, add 0.0005 μg of diethylstilbestrol, hexestrol, diethylstilbestrol and bisphenol A to one sample, and add 0.0005 μg of diethylstilbestrol to the other. Add 0.0002 μg of diethylstilbestrol, hexestrol, diethylstilbestrol, and bisphenol A to a sample, then put the sample in the first centrifuge bottle with a stopper, add 10-20ml of acetonitrile to homogenize, and centrifuge at 2000rpm for 5min , the supernatant was transferred to the second centrifuge bottle with a stopper, 10-20ml of acetonitrile was added to the first centrifuge bottle, after shaking well, centrifuged at 2000rpm for 5min, the supernatant was combined in the second centrifuge bottle, and the solid sample was discarded. sample; in the second centrifuge bottle, first add 16ml of n-hexane and 4ml of dichloromethane, after shaking well, centrifuge at 2000rpm for 3min, and discard the upper layer of n-hexane ph...

Embodiment 2

[0014] Take 5 g of chopped and homogenized carp muscle, without adding diethylstilbestrol, hexestrol, diethylstilbestrol and bisphenol A, the extraction method and test method are the same as in Example 1, and finally the content of diethylstilbestrol in carp muscle is 0.008 μg, diethylstilbestrol 0.011μg, the content of diethylstilbestrol in meat is about 2μg / kg, and the content of diethylstilbestrol is about 2.7μg / kg.

Embodiment 3

[0016] Take 5g of minced and homogenized pork muscle, without adding diethylstilbestrol, hexestrol, dienestrol and bisphenol A, the extraction method and test method are the same as in Example 1, and finally the muscle contains hexane 0.02 μg of estrol, 0.032 μg of bisphenol A, the content of diethylstilbestrol in meat is about 5 μg / kg, and the content of bisphenol A is about 8 μg / kg.

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PUM

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Abstract

The invention discloses a method for extracting diethylstilbestrol, hexestrol, dienestrol and / or bisphenol A from animal muscle. The method comprises the following steps of: separating a non-polar substance through acetonitrile extraction with normal hexane and dichloromethane in a volume ratio of 4:1, and further dissolving synthetic glucocorticoids in an organic phase with normal hexane and dichloromethane in a volume ratio of 1:2; and separating the polar substance in the water phase without purification of a solid-phase extraction column. According to the method, the extraction cost can be reduced, the purity of the diethylstilbestrol, hexestrol, dienestrol and / or bisphenol A extracts can be high, inorganic and organic impurities are few, an internal standard gas chromatography-mass spectrometry can be used for detecting, the detection limit is low, and the detection limit of each synthetic glucocorticoid is about 0.05mu g / kg, and the quantitative detection has few errors.

Description

technical field [0001] The invention relates to a method for extracting artificially synthesized hormones in animals, in particular to a method for extracting diethylstilbestrol, hexestrol, dienestrol and / or bisphenol A from animal muscles. Background technique [0002] At present, the breeding industry of poultry, fish, shrimp or crab is well developed. Although growth-promoting hormones are not allowed to be used in animal breeding, due to the remarkable growth-promoting effect of artificial synthetic hormones such as diethylstilbestrol and the wide use of bisphenol A, people can touch it everywhere. The environmental pollutants that have been detected, animal-derived foods may have these estrogen-like residues, and human health will be affected after consumption. Therefore, it is particularly important to detect the residual amount of estrogens such as diethylstilbestrol in animal tissues to ensure the safety of animal-derived food. There are various methods for detecti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06
Inventor 钟惠英柴丽月朱励华柳海
Owner NINGBO ACAD OF OCEAN & FISHERY